引起[植物名称未给出]叶斑病的病原菌鉴定及其对五种杀菌剂的敏感性

Identification of the Pathogen Causing Leaf Spot in and Its Sensitivity to Five Fungicides.

作者信息

Liu Yu, Yao Qiuyu, Liang Shuang, Li Cheng, Chen Xiangsheng, Li Zhong

机构信息

Key Laboratory of Agricultural Microbiology, College of Agriculture, Guizhou University, Guiyang 550025, China.

Provincial Key Laboratory of Insect Resources Development and Utilization, Guizhou University, Guiyang 550025, China.

出版信息

Pathogens. 2022 Dec 1;11(12):1454. doi: 10.3390/pathogens11121454.

DOI:10.3390/pathogens11121454

PMID:36558787

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9783861/

Abstract

Jacq. is an important, globally cultivated ornamental plant. In August 2021, a leaf spot disease was observed in zinnia in Shibing County, Guizhou, China, with an incidence of approximately 60%. Pathogens were isolated and purified from the infected leaves by tissue isolation, and pathogen strain BRJ2 was confirmed as the pathogen causing the leaf spot. Based on morphology and , , and sequence analyses, the pathogen was identified as (McLennan and Hoëtte). The mycelial growth rate method was used to determine the in vitro toxicity of five fungicides to the pathogen. The results showed that 10% difenoconazole provided the strongest inhibitory effect on , with a concentration for 50% of maximal effect (EC) of 0.0658 mg/L; 75% trifloxystrobin·tebuconazole had the second greatest effect, with an EC of 0.1802 mg/L. This study provides the first report that caused leaf spot disease in and provides important guidance for the effective prevention and control of this disease in Guizhou.

摘要

百日草是一种重要的全球广泛种植的观赏植物。2021年8月，在中国贵州施秉县的百日草中观察到叶斑病，发病率约为60%。通过组织分离从感染叶片中分离并纯化病原体，病原体菌株BRJ2被确认为导致叶斑病的病原体。基于形态学以及……和……序列分析，该病原体被鉴定为……（麦克伦南和霍埃特）。采用菌丝生长速率法测定了5种杀菌剂对该病原体的体外毒力。结果表明，10%苯醚甲环唑对……的抑制作用最强，最大效应浓度（EC）50%为0.0658mg/L；75%肟菌·戊唑醇效果次之，EC为0.1802mg/L。本研究首次报道……在百日草中引起叶斑病，为贵州有效防控该病提供了重要指导。