越南献血者中隐匿性乙型肝炎感染风险较低。
Low Risk of Occult Hepatitis B Infection among Vietnamese Blood Donors.
作者信息
Tung Tran Thanh, Schmid Jürgen, Nghia Vu Xuan, Cao Le Chi, Linh Le Thi Kieu, Rungsung Ikrormi, Sy Bui Tien, My Truong Nhat, The Nguyen Trong, Hoan Nghiem Xuan, Meyer Christian G, Wedemeyer Heiner, Kremsner Peter G, Toan Nguyen Linh, Song Le Huu, Bock C-Thomas, Velavan Thirumalaisamy P
机构信息
Institute of Tropical Medicine, University of Tübingen, 72074 Tübingen, Germany.
Vietnamese-German Center for Medical Research (VG-CARE), Hanoi 10000, Vietnam.
出版信息
Pathogens. 2022 Dec 13;11(12):1524. doi: 10.3390/pathogens11121524.
Occult hepatitis B infection (OBI) is characterized by the presence of low levels of hepatitis B virus (HBV) DNA and undetectable HBsAg in the blood. The prevalence of OBI in blood donors in Asia ranges from 0.013% (China) to 10.9% (Laos), with no data available from Vietnam so far. We aimed to investigate the prevalence of OBI among Vietnamese blood donors. A total of 623 (114 women and 509 men) HBsAg-negative blood donors were screened for anti-HBc and anti-HBs by ELISA assays. In addition, DNA from sera was isolated and nested PCR was performed for the HBV surface gene (S); a fragment of the S gene was then sequenced in positive samples. The results revealed that 39% ( 242) of blood donors were positive for anti-HBc, and 70% ( 434) were positive for anti-HBs, with 36% ( 223) being positive for both anti-HBc and anti-HBs. In addition, 3% of blood donors ( 19) were positive for anti-HBc only, and 34% ( 211) had only anti-HBs as serological marker. A total of 27% ( 170) were seronegative for any marker. Two of the blood donors (0.3%) were OBI-positive and sequencing revealed that HBV sequences belonged to HBV genotype B, which is the predominant genotype in Vietnam.
隐匿性乙型肝炎感染（OBI）的特征是血液中存在低水平的乙型肝炎病毒（HBV）DNA且检测不到HBsAg。亚洲献血者中OBI的患病率从0.013%（中国）到10.9%（老挝）不等，目前尚无越南的相关数据。我们旨在调查越南献血者中OBI的患病率。通过酶联免疫吸附测定法（ELISA）对623名（114名女性和509名男性）HBsAg阴性献血者进行抗-HBc和抗-HBs筛查。此外，从血清中提取DNA，并对HBV表面基因（S）进行巢式聚合酶链反应（PCR）；然后对阳性样本中的S基因片段进行测序。结果显示，39%（242名）献血者抗-HBc呈阳性，70%（434名）抗-HBs呈阳性，36%（223名）抗-HBc和抗-HBs均呈阳性。此外，3%（19名）献血者仅抗-HBc呈阳性，34%（211名）仅以抗-HBs作为血清学标志物。共有27%（170名）献血者所有标志物均呈血清学阴性。两名献血者（0.3%）为OBI阳性，测序显示HBV序列属于HBV B基因型，这是越南的主要基因型。
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