Murphy J R, Lefford M J
Infect Immun. 1978 Dec;22(3):798-803. doi: 10.1128/iai.22.3.798-803.1978.
Random-bred mice were immunized with a nonliving antigen prepared from mixed-blood forms of Plasmodium berghei, strain NYU-2, in combination with Corynebacterium parvum and/or living BCG. A high proportion of intravenously immunized mice survived virulent challenge, but subcutaneous vaccination was less effective. Vaccinated mice developed a patent infection after challenge similar to that observed in normal controls. However, between days 12 to 20 postchallenge, infections in some vaccinated mice became subpatent, whereas infections in all normal controls progressed until death. The incidence of recrudescent infection was low and, eventually, a state of sterile immunity was established. The capacity of vaccinated mice to withstand P. berghei challenge was sustained at a fairly stable level for the 6-month period of observation. Mice that had survived a primary infection with P. berghei almost completely suppressed a second and larger challenge with the same organism.
将随机繁殖的小鼠用由伯氏疟原虫NYU - 2株的混合血液形式制备的非活性抗原与短小棒状杆菌和/或活卡介苗联合免疫。高比例经静脉免疫的小鼠在受到强毒攻击后存活，但皮下接种效果较差。接种疫苗的小鼠在受到攻击后出现明显感染，类似于在正常对照中观察到的情况。然而，在攻击后第12至20天之间，一些接种疫苗的小鼠的感染变为隐性感染，而所有正常对照中的感染则持续发展直至死亡。复发感染的发生率较低，最终建立了无菌免疫状态。在6个月的观察期内，接种疫苗的小鼠抵御伯氏疟原虫攻击的能力维持在相当稳定的水平。曾在初次感染伯氏疟原虫中存活的小鼠几乎完全抑制了用同一病原体进行的第二次更大剂量的攻击。
