150千赫中频电磁辐射对雄性大鼠生育指标的影响。

Effects of 150 kHz intermediate frequency electromagnetic radiation on fertility indicators in male rats.

作者信息

Sundaram Venkatesan, Mohammed Stephanie, Zyuzikov Nikolay

机构信息

Department of Basic Veterinary Sciences, School of Veterinary Medicine, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago.

Department of Physics, Faculty of Science and Technology, The University of the West Indies, St. Augustine, Trinidad and Tobago.

出版信息

Heliyon. 2022 Dec 9;8(12):e12228. doi: 10.1016/j.heliyon.2022.e12228. eCollection 2022 Dec.

DOI:10.1016/j.heliyon.2022.e12228

PMID:36578412

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9791864/

Abstract

BACKGROUND

The present study aimed to evaluate the effects of whole-body exposure to 150 kHz Intermediate-frequency electromagnetic radiation (IF EMR) on fertility indicators of male rats since human exposure to this frequency has increased in recent years. Fourteen adult male Sprague-Dawley rats were used in this study. The rats were randomly divided into a control and an EMR group (n = 7/group). The EMR group was continuously irradiated with 150 kHz EMR for 8 weeks. Male fertility indicators, body mass, testicular mass, rectal temperature, testicular histology, histometry, sperm analysis, and serum gonadotrophic hormone levels were evaluated.

RESULTS

The study showed no negative effect on body mass (grams) (323.78 ± 37.09 to 305.09 ± 26.36; p = 0.72), rectal temperature (Control: 34.5 °C-35.8 °C; EMR: 34.4 °C-36.1 °C; p < 0.05), and testicular histology. There were significant reductions in left and right testicular mass (1.04 ± 0.10 to 0.96 ± 0.32: p = 0.03 and 1.02 ± 0.08 to 0.96 ± 0.35 p = 0.04, respectively), interstitial cell count/1000 μm (5.33 ± 0.56 to 4.47 ± 0.48; p = 0.01), sperm motility trajectories (p = 0.05) and sperm distal cytoplasmic droplet (%) (2.27 ± 2.28 to 6.84 ± 5.01; p = 0.05). A significant increase in follicle-stimulating hormone levels was observed (13.44 ± 6.38 IU/ml to 26.96 ± 8.07 IU/ml; p = 0.01).

CONCLUSIONS

Most male fertility parameters of rats in the present study were not affected by 8 weeks of whole-body exposure to 150 kHz EMR. However, significant decreases in testicular mass, interstitial cell count/1000 μm, sperm motility trajectories, and distal cytoplasmic droplets were observed, as well as an increase in FSH level.

摘要

背景

近年来，人类接触150千赫中频电磁辐射（IF EMR）的情况有所增加，本研究旨在评估全身暴露于该频率的电磁辐射对雄性大鼠生育指标的影响。本研究使用了14只成年雄性斯普拉格-道利大鼠。将大鼠随机分为对照组和电磁辐射组（每组n = 7）。电磁辐射组连续8周接受150千赫电磁辐射照射。评估了雄性生育指标、体重、睾丸重量、直肠温度、睾丸组织学、组织测量、精子分析和血清促性腺激素水平。

结果

研究表明，对体重（克）（从323.78±37.09降至305.09±26.36；p = 0.72）、直肠温度（对照组：34.5℃ - 35.8℃；电磁辐射组：34.4℃ - 36.1℃；p < 0.05）和睾丸组织学没有负面影响。左右睾丸重量显著降低（分别从1.04±0.10降至0.96±0.32：p = 0.03和从1.02±0.08降至0.96±0.35，p = 0.04），间质细胞计数/1000μm（从5.33±0.56降至4.47±0.48；p = 0.01），精子运动轨迹（p = 0.05）以及精子远端细胞质滴（%）（从2.27±2.28升至6.84±5.01；p = 0.05）。观察到促卵泡激素水平显著升高（从13.44±6.38国际单位/毫升升至26.96±8.07国际单位/毫升；p = 0.01）。