Asghari Golaleh, Yuzbashian Emad, Nikparast Ali, Najd Hassan Bonab Leila, Mahdavi Maryam, Daneshpour Maryam S, Hosseinpanah Farhad, Mirmiran Parvin
Department of Clinical Nutrition and Dietetics, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Nutrition and Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Front Nutr. 2022 Dec 12;9:1061496. doi: 10.3389/fnut.2022.1061496. eCollection 2022.
The rs2282679 polymorphism in the vitamin D binding protein (DBP) gene may influence the response to vitamin D supplementation. Therefore, we examine the effect of 1-year vitamin D supplementation on vitamin D deficiency (VDD) with the interaction of rs2282679 polymorphism in overweight and obese children and adolescents.
The participants ( = 300) were part of a randomized controlled trial who received a daily supplement of either 1,000 or 2,000 IU or four supplements of 1,000 IU weekly (equal to 600 IU daily) of vitamin D for 12 months. Genotyping was performed using amplification refractory mutation system polymerase chain reaction (ARMS-PCR).
The mean of 25(OH)D values at baseline for participants with the TT, TG, and GG genotypes were 15.4, 14.4, and 10.8 ng/mL, respectively, and were not different between the three genotype groups ( = 0.062). A significant reduction in VDD was observed after vitamin D supplementation with dosages of 1,000 or 2,000 IU compared to 600 IU. No significant association of genotypes with risk of VDD was observed in each intervention group after vitamin D supplementation, except, that individuals with TG genotype showed a higher risk of VDD compared to those with TT genotype in the 2,000 IU group after 6 months of supplementation [odds ratio (95% CI): 6.94; 1.30-37.02]. We observed no interaction between time duration, three genotypes, and dosages with serum 25(OH)D, calcium, phosphorus, alkaline phosphatase, and parathyroid hormone levels.
Response to vitamin D supplementation by three doses of 600, 1,000, and 2,000 IU could not be affected by rs2282679 polymorphism during 12 months in overweight and obese children and adolescents.
维生素D结合蛋白（DBP）基因中的rs2282679多态性可能会影响维生素D补充治疗的反应。因此，我们研究了超重和肥胖儿童及青少年中rs2282679多态性与维生素D补充治疗的相互作用对维生素D缺乏（VDD）的影响。
参与者（n = 300）是一项随机对照试验的一部分，他们每天补充1000或2000 IU维生素D，或每周补充四次1000 IU（相当于每天600 IU）维生素D，持续12个月。使用扩增阻滞突变系统聚合酶链反应（ARMS-PCR）进行基因分型。
TT、TG和GG基因型参与者的基线25(OH)D值平均值分别为15.4、14.4和10.8 ng/mL，三组基因型之间无差异（P = 0.062）。与600 IU相比，补充1000或2000 IU维生素D后，VDD显著降低。补充维生素D后，各干预组中未观察到基因型与VDD风险的显著关联，不过，在补充6个月后，2000 IU组中，TG基因型个体与TT基因型个体相比，VDD风险更高[比值比（95%可信区间）：6.94；1.30 - 37.02]。我们未观察到时间、三种基因型和剂量与血清25(OH)D、钙、磷、碱性磷酸酶和甲状旁腺激素水平之间存在相互作用。
在超重和肥胖儿童及青少年中，12个月内，600、1000和2000 IU三种剂量的维生素D补充治疗反应不受rs2282679多态性的影响。
