Department of Orthopedic Surgery, Seoul National University Hospital, Seoul, Republic of Korea.
Department of Pathology, Seoul National University Bundang Hospital, Seongnam si, Republic of Korea.
Gerontology. 2023;69(5):526-532. doi: 10.1159/000528160. Epub 2022 Dec 30.
Animal studies suggest that advanced glycation end products (AGEs) and their interaction with receptor for AGEs (RAGE) are involved in sarcopenia, but their relationship in human skeletal muscles has yet to be elucidated. We aimed to determine whether RAGE expression in human skeletal muscle is associated with serum AGE levels and sarcopenia-related changes.
We retrospectively reviewed 33 consecutive women (mean age, 65 years) with distal radius fracture who had consented to donate a sample of forearm muscle for research purposes, which was taken during surgical fracture repair. The muscle RAGE expression was measured with immunohistochemistry staining and serum AGE levels using ELISA method. We compared RAGE expression and AGE levels in patients with and without sarcopenia. We also correlated RAGE expression with such clinical parameters as demographic factors, as well as sarcopenia-related changes, including grip strength, appendicular skeletal muscle mass, and muscle cross-sectional area (CSA) ratios.
Twelve patients (36%) were diagnosed with sarcopenia. They had a significantly higher RAGE expression (p = 0.044) and AGE level (p < 0.001) than those without sarcopenia. The RAGE expression correlated significantly with a high AGE level (r = 0.510, p = 0.011) and correlated inversely with a muscle CSA ratio (r = -0.696, p < 0.001).
This study shows that RAGE expression increases in sarcopenic patient skeletal muscles. This expression also correlates positively with serum AGE levels and inversely with muscle CSA ratios. Further studies are necessary to determine whether targeting RAGEs can be a therapeutic option for sarcopenia.
动物研究表明，晚期糖基化终产物（AGEs）及其与 AGE 受体（RAGE）的相互作用与肌肉减少症有关，但它们在人类骨骼肌中的关系尚未阐明。我们旨在确定人类骨骼肌中 RAGE 的表达是否与血清 AGE 水平和与肌肉减少症相关的变化有关。
我们回顾性分析了 33 名连续的桡骨远端骨折女性患者（平均年龄 65 岁），她们同意在手术骨折修复期间捐献前臂肌肉样本用于研究。使用免疫组织化学染色法测量肌肉 RAGE 表达，使用 ELISA 法测量血清 AGE 水平。我们比较了有和没有肌肉减少症的患者的 RAGE 表达和 AGE 水平。我们还将 RAGE 表达与人口统计学因素等临床参数以及与肌肉减少症相关的变化（包括握力、四肢骨骼肌质量和肌肉横截面积（CSA）比值）进行了相关性分析。
12 名患者（36%）被诊断为肌肉减少症。与没有肌肉减少症的患者相比，他们的 RAGE 表达（p = 0.044）和 AGE 水平（p < 0.001）显著更高。RAGE 表达与高 AGE 水平显著相关（r = 0.510，p = 0.011），与肌肉 CSA 比值呈负相关（r = -0.696，p < 0.001）。
本研究表明，RAGE 在肌肉减少症患者的骨骼肌中表达增加。这种表达还与血清 AGE 水平呈正相关，与肌肉 CSA 比值呈负相关。需要进一步的研究来确定是否可以将靶向 RAGEs 作为肌肉减少症的治疗选择。
