血清卷曲螺旋结构域蛋白 25（CCDC25）水平升高可作为胆管癌的诊断标志物，但不能用于其他癌症。
High Level of Serum Coiled-coil Domain Containing 25 (CCDC25) as a Diagnostic Marker for Cholangiocarcinoma But Not for Other Cancers.
机构信息
Centre of Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
Cholangiocarcinoma Research Institute, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
出版信息
In Vivo. 2023 Jan-Feb;37(1):294-303. doi: 10.21873/invivo.13079.
BACKGROUND/AIM: Recently, we reported that coiled-coil domain containing 25 (CCDC25) protein is elevated in the sera of patients with cholangiocarcinoma (CCA) and is suggested to be a diagnostic biomarker for CCA. This study aimed to examine whether serum CCDC25 level can be a unique biomarker for CCA. Bioinformatic analyses using Human Protein Atlas (HPA) database and Gene Expression Profiling Interactive Analysis 2 (GEPIA2) indicated that CCDC25 protein and mRNA are expressed not only in CCA but also in other cancers, such as colorectal cancer (CRC), breast cancer (BC), and hepatocellular carcinoma (HCC), all of which are the top 5 cancers highly prevalent in Thailand.
MATERIALS AND METHODS
Using a quantitative dot blot assay, serum CCDC25 levels were measured for 30 healthy controls (HC), 34 CRC, 42 BC, 43 HCC, and 83 CCA.
RESULTS
The serum CCDC25 levels of CCA patients (0.193±0.039 ng/μl) were significantly higher than those of CRC (0.019±0.006 ng/μl), BC (0.036±0.015 ng/μl), HCC (0.035±0.016 ng/μl), and higher than those of HC (0.012±0.003 ng/μl). The serum CCDC25 level can discriminate CCA from the HC, CRC, BC, and HCC with a sensitivity of 100, 99, 94, and 94%, respectively, and specificity of 100, 100, 98, and 95%, respectively.
CONCLUSION
CCDC25 is a candidate diagnostic biomarker for CCA.
背景/目的：最近，我们报道卷曲螺旋结构域蛋白 25（CCDC25）在胆管癌（CCA）患者的血清中升高，并被认为是 CCA 的诊断生物标志物。本研究旨在研究血清 CCDC25 水平是否可作为 CCA 的独特生物标志物。使用人类蛋白质图谱（HPA）数据库和基因表达谱交互分析 2（GEPIA2）的生物信息学分析表明，CCDC25 蛋白和 mRNA 不仅在 CCA 中表达，而且在其他癌症中也表达，如结直肠癌（CRC）、乳腺癌（BC）和肝细胞癌（HCC），这些都是在泰国高发的前 5 种癌症。
材料和方法
使用定量斑点印迹法测量 30 名健康对照（HC）、34 名 CRC、42 名 BC、43 名 HCC 和 83 名 CCA 患者的血清 CCDC25 水平。
结果
CCA 患者的血清 CCDC25 水平（0.193±0.039 ng/μl）明显高于 CRC（0.019±0.006 ng/μl）、BC（0.036±0.015 ng/μl）、HCC（0.035±0.016 ng/μl），也高于 HC（0.012±0.003 ng/μl）。血清 CCDC25 水平可以区分 CCA 与 HC、CRC、BC 和 HCC，其敏感性分别为 100%、99%、94%和 94%，特异性分别为 100%、100%、98%和 95%。
结论
CCDC25 是 CCA 的候选诊断生物标志物。
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