Endoh Tamaki, Takahashi Shuntaro, Sugimoto Naoki
Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 7-1-20 Minatojima-minamimachi, Kobe, 650-0047, Japan.
Graduate School of Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, 7-1-20 Minatojima-minamimachi, Kobe, 650-0047, Japan.
Chem Commun (Camb). 2023 Jan 19;59(7):872-875. doi: 10.1039/d2cc05858h.
Replication of RNA viruses is catalysed by virus-specific polymerases, which can be targets of therapeutic strategies. In this study, we used a selection strategy to identify endogenous RNAs from a transcriptome library derived from lung cells that interact with the RNA-dependent RNA polymerase (RdRp) of SARS-CoV-2. Some of the selected RNAs weakened the activity of RdRp by forming G-quadruplexes. These results suggest that certain endogenous RNAs, which potentially form G-quadruplexes, can reduce the replication of viral RNAs.
RNA病毒的复制由病毒特异性聚合酶催化，这些聚合酶可能成为治疗策略的靶点。在本研究中，我们采用一种筛选策略，从源自肺细胞的转录组文库中鉴定与严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的RNA依赖性RNA聚合酶（RdRp）相互作用的内源性RNA。一些筛选出的RNA通过形成G-四链体削弱了RdRp的活性。这些结果表明，某些可能形成G-四链体的内源性RNA可减少病毒RNA的复制。
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