提取物负载壳聚糖纳米粒的合成、表征及神经保护作用。
Synthesis characterisation and neuroprotectivity of extract loaded chitosan nanoparticles.
机构信息
Department of Bioengineering, Yildiz Technical University, Istanbul, Turkey.
Department of Biomedical Engineering, Istanbul Gelisim University, Istanbul, Turkey.
出版信息
J Microencapsul. 2023 Jan;40(1):29-36. doi: 10.1080/02652048.2023.2167012. Epub 2023 Jan 25.
AIM
extract (SME) possesses neuroprotective potency through its high antioxidant content. We attempted to increase the effectiveness of SME by encapsulating them in chitosan. Neuroprotective potency of SME and SME-loaded chitosan nanoparticles (SME-CNPs) were shown in SH-SY5Y cell line against HO-induced oxidative stress.
METHODS
We produced CNPs and SME-CNPs by ionic gelation method and properly determined their physical characteristics. Encapsulation efficiency, loading capacity, and release tests were performed for SME-CNPs. The neurotoxicity and neuroprotective efficiency in SH-SY5Y cell line against HO was also investigated.
RESULTS
The size of SME-CNPs was 168.2 ± 11.12 nm with zeta potential 10.6 ± 1.0 mV. The encapsulation efficiency and loading capacity were successfully achieved at 96.6% and 1.89% respectively. SME and SME-CNPs improved cell viability higher than 80%, and SME-CNPs exhibited significant neuroprotective effects against HO damage.
CONCLUSIONS
It was concluded that SME and SME-CNPs highly prevent damage caused by HO and reduce cell damage by their neuroprotective effects.
目的
由于具有高抗氧化含量，SME 具有神经保护作用。我们试图通过将其包封在壳聚糖中来提高 SME 的有效性。在 HO 诱导的氧化应激下，我们在 SH-SY5Y 细胞系中显示了 SME 和负载 SME 的壳聚糖纳米颗粒（SME-CNPs）的神经保护作用。
方法
我们通过离子凝胶化法制备 CNPs 和 SME-CNPs，并适当确定了它们的物理特性。对 SME-CNPs 进行了包封效率、载药量和释放测试。还研究了 SME-CNPs 在 SH-SY5Y 细胞系中对 HO 的神经毒性和神经保护效率。
结果
SME-CNPs 的粒径为 168.2±11.12nm，zeta 电位为 10.6±1.0mV。包封效率和载药量分别成功达到 96.6%和 1.89%。SME 和 SME-CNPs 使细胞活力提高了 80%以上，并且 SME-CNPs 对 HO 损伤表现出显著的神经保护作用。
结论
可以得出结论，SME 和 SME-CNPs 通过其神经保护作用，高度预防 HO 引起的损伤并减少细胞损伤。
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