Chloramphenicol (CAP) is a widely used antibiotic for the treatment of sick animals owing to its potent action and low cost. However, the accumulation of CAP in the human body can cause irreversible aplastic anemia and hematopoietic toxicity. Accordingly, development of various analytical techniques for the rapid detection of CAP in animal products and the related processed foods is necessary. Among these analytical techniques, electrochemical and optical sensors offer many advantages for CAP detection, including high sensitivity, simple operation and fast analysis speed. In this review, we summarize recent application of carbon nanomaterials, metal nanoparticles, metal oxide nanoparticles and metal organic framework in the development of electrochemical and optical sensors for CAP detection (2010-2022). Based on the advantages and disadvantages of nanomaterials, electrochemical and optical sensors are summarized in this review. The preparation and synthesis of electrochemical and optical sensors and nanomaterials in the field of rapid detection are prospected.