Zeng Liming, Zeng Liwang, Wang Yu, Xie Zhengnan, Zhao Minhua, Chen Jie, Ye Xiaoxue, Tie Weiwei, Li Meiying, Shang Sang, Tian Libo, Zeng Jian, Hu Wei
Sanya Nanfan Research Institute of Hainan University, Sanya, Hainan, China.
Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, School of Horticulture, Hainan University, Haikou, China.
Genetica. 2023 Apr;151(2):87-96. doi: 10.1007/s10709-023-00178-w. Epub 2023 Jan 18.
Plant hormone abscisic acid (ABA) plays an important role in plant growth, development and response to biotic / abiotic stressors. Thus, it is necessary to investigate the crucial genes associated with ABA synthesis. Currently, the carotenoid cleavage oxygenases (CCOs) family that function as the key step for ABA synthesis are not well understood in banana. In this study, 13 MaCCO genes and 12 MbCCO genes, divided into NCED subgroup and CCD subgroup, were identified from the banana genome, and their evolutionary relationship, protein motifs, and gene structures were also determined. Transcriptomic analysis suggested the involvement of CCO genes in banana development, ripening, and response to abiotic and biotic stressors, and homologous gene pairs showed homoeologue expression bias in the A or B subgenome. Our results identified MaNCED3A, MaCCD1, and MbNCED3B as the genes with the highest expression during fruit development and ripening. MaNCED5 / MbNCED5 and MaNCED9A might respond to abiotic stress, and MaNCED3A, 3B, 6 A, 9 A, and MbNCED9A showed transcriptional changes that could be a response to Foc4 infection. These findings may contribute to the characterization of key enzymes involved in ABA biosynthesis, as well as to identify potential targets for the genetic improvement of banana.
植物激素脱落酸（ABA）在植物生长、发育以及对生物/非生物胁迫的响应中发挥着重要作用。因此，有必要研究与ABA合成相关的关键基因。目前，作为ABA合成关键步骤的类胡萝卜素裂解双加氧酶（CCOs）家族在香蕉中的情况尚不清楚。在本研究中，从香蕉基因组中鉴定出13个MaCCO基因和12个MbCCO基因，它们被分为NCED亚组和CCD亚组，同时还确定了它们的进化关系、蛋白质基序和基因结构。转录组分析表明，CCO基因参与了香蕉的发育、成熟以及对非生物和生物胁迫的响应，并且同源基因对在A或B亚基因组中表现出同源基因表达偏向性。我们的结果确定MaNCED3A、MaCCD1和MbNCED3B为果实发育和成熟过程中表达量最高的基因。MaNCED5/MbNCED5和MaNCED9A可能对非生物胁迫有响应，而MaNCED3A、3B、6A、9A和MbNCED9A的转录变化可能是对尖孢镰刀菌4号生理小种（Foc4）感染的响应。这些发现可能有助于阐明参与ABA生物合成的关键酶的特性，以及为香蕉的遗传改良确定潜在靶点。
