淋巴细胞性脉络丛脑膜炎病毒特异性T细胞及LCMV病毒滴度的定量分析。

Quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers.

作者信息

Grusdat Melanie, Dostert Catherine, Brenner Dirk

机构信息

Experimental and Molecular Immunology, Department of Infection and Immunity, Luxembourg Institute of Health, Esch-sur-Alzette, Luxembourg; Immunology & Genetics, Luxembourg Centre for Systems Biomedicine, University of Luxembourg, Esch-sur-Alzette, Luxembourg; Odense Research Center for Anaphylaxis (ORCA), Department of Dermatology and Allergy Center, Odense University Hospital, University of Southern Denmark, Odense, Denmark.

出版信息

Methods Cell Biol. 2023;173:121-131. doi: 10.1016/bs.mcb.2022.03.003. Epub 2022 May 11.

DOI:10.1016/bs.mcb.2022.03.003

PMID:36653079

Abstract

Lymphocytic choriomeningitis virus (LCMV) is a frequently used animal model to study immune responses against acute and chronic viral infections. LCMV is a non-cytopathic virus, but destruction of infected cells by cytotoxic T lymphocytes (CTLs) can lead to severe damage of tissues. Virus-specific T cell responses have to be balanced. A low virus load leads to a strong T cell response and subsequently to viral control. In contrast, a high viral titer is associated with T cell exhaustion and chronic viral infections. During an intermediate LCMV viral load CD8 T cells can cause immunopathology, which can have detrimental outcomes. The LCMV infection model offers the opportunity to study virus-specific CD4 and CD8 T cell responses during chronic and acute infections by quantifying LCMV-specific T cells by tetramer staining and measuring cytokine production and viral titers in different organs.

摘要

淋巴细胞性脉络丛脑膜炎病毒（LCMV）是一种常用于研究针对急性和慢性病毒感染的免疫反应的动物模型。LCMV是一种非细胞病变病毒，但细胞毒性T淋巴细胞（CTL）对受感染细胞的破坏可导致组织严重损伤。病毒特异性T细胞反应必须保持平衡。低病毒载量会导致强烈的T细胞反应，进而实现病毒控制。相反，高病毒滴度与T细胞耗竭和慢性病毒感染相关。在中等LCMV病毒载量期间，CD8 T细胞可引起免疫病理，这可能产生有害后果。LCMV感染模型提供了一个机会，通过四聚体染色定量LCMV特异性T细胞，并测量不同器官中的细胞因子产生和病毒滴度，来研究慢性和急性感染期间病毒特异性CD4和CD8 T细胞反应。