Department of Pathology, Shri Guru Ram Rai Institute of Medical and Health Sciences, Dehradun, Uttarakhand, India.
Indian J Pathol Microbiol. 2023 Jan-Mar;66(1):63-69. doi: 10.4103/ijpm.ijpm_656_21.
The present study evaluates the immunoexpression of p16 and Ki-67 in cervical squamous intraepithelial lesion (SIL) and carcinomas and correlates their expression with clinicopathological features and HPV-DNA status.
A total 36 included cases of SIL and squamous cell carcinoma (SCC) were subjected to p16 and Ki-67 immunostaining. p16 staining was evaluated depending on grading, distribution, localization pattern, intensity and IHC score. Ki-67 expression was graded based on percentage of positive cells.
Incidence of HSIL and SCC cases was found to be significantly increased with parity > 5. p16 grade III diffuse nucleocytoplasmic immunostaining was observed in 62.5% LSIL, 80% HSIL and 87% SCC cases. Significant association of p16 staining intensity, IHC score and Ki-67 indices was noted with increasing grades of SILs and carcinomas.
Our experience indicates that a combination of p16 and Ki-67 immunostaining may be useful to determine the severity of dysplastic change.
本研究评估了 p16 和 Ki-67 在宫颈鳞状上皮内病变（SIL）和癌中的免疫表达，并将其表达与临床病理特征和 HPV-DNA 状态相关联。
对 36 例 SIL 和鳞状细胞癌（SCC）病例进行了 p16 和 Ki-67 免疫染色。p16 染色根据分级、分布、定位模式、强度和 IHC 评分进行评估。Ki-67 的表达根据阳性细胞的百分比进行分级。
发现产次>5 的 HSIL 和 SCC 病例的发生率显著增加。62.5%的 LSIL、80%的 HSIL 和 87%的 SCC 病例中观察到 p16 级 III 弥漫核浆免疫染色。p16 染色强度、IHC 评分和 Ki-67 指数与 SIL 和癌的分级增加呈显著相关。
我们的经验表明，p16 和 Ki-67 免疫染色的组合可能有助于确定发育不良变化的严重程度。
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