犬埃立克体：基于 p28 和 trp36 基因的分子特征和遗传多样性。
Ehrlichia canis: Molecular characterization and genetic diversity based on the p28 and trp36 genes.
机构信息
Parasitology Research Laboratory (PRL), Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand.
Parasitology Research Laboratory (PRL), Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand; Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
出版信息
Res Vet Sci. 2023 Feb;155:88-102. doi: 10.1016/j.rvsc.2022.11.013. Epub 2022 Dec 13.
Ehrlichia canis is a common tick-borne intracellular pathogen causing canine monocytic ehrlichiosis (CME) in dogs worldwide. The aims of this study were to investigate the genetic diversity and antigenicity of E. canis based on the p28 and trp36 genes in dogs in Thailand. The E. canis p28 and trp36 genes were amplified by the polymerase chain reaction (PCR) and cloned for sequencing and bioinformatic analyses. 36% (44/120) of dog blood samples were positive for E. canis DNA consisting of p28 (31%, 14/44) and trp36 (69%, 30/44) genes with 792 and 882 bp of PCR products size, respectively. The E. canis TRP36 from all Thailand sequences exhibited encoded nine amino acids (TEDSVSAPA) with 11 copies of tandem repeats along the sequences. The phylogenetic trees of E. canis, using the p28 and trp36 genes, exhibited that the Thailand isolates fell into two clades and one clade with similarity ranging from 55.95 to 100% and 100%, respectively. The results of diversity analysis revealed 10 and 20 haplotypes of the p28 and trp 36 genes, respectively. The entropy analysis of the p28 and trp36 nucleic acid sequences showed 442 and 1321 high entropy peaks respectively, whereas those of the P28 and TRP36 amino acid sequences showed 477 and 388 high entropy peaks, respectively. For B-cell epitopes analysis, the conserved amino acid of P28 and TRP36 sequences has been also demonstrated. Therefore, the results could be utilized to improve the understanding of phylogenetic relationship, genetic diversity and antigenicity of E. canis Thailand isolates.
犬埃立克体是一种常见的蜱传细胞内病原体，可引起全球犬单核细胞埃立克体病（CME）。本研究旨在基于泰国犬的 p28 和 trp36 基因，研究犬埃立克体的遗传多样性和抗原性。通过聚合酶链反应（PCR）扩增埃立克体的 p28 和 trp36 基因，并进行测序和生物信息学分析。36%（44/120）的犬血样检测到埃立克体 DNA，包括 p28（31%，14/44）和 trp36（69%，30/44）基因，PCR 产物大小分别为 792 和 882bp。来自泰国的所有埃立克体 TRP36 序列显示编码 9 个氨基酸（TEDSVSAPA），序列中存在 11 个串联重复。基于 p28 和 trp36 基因构建的埃立克体系统发育树显示，泰国分离株分为两个分支，一个分支与相似性为 55.95%至 100%和 100%的分支聚类。多样性分析结果显示，p28 和 trp36 基因分别有 10 个和 20 个单倍型。p28 和 trp36 核酸序列的熵分析分别显示了 442 个和 1321 个高熵峰，而 p28 和 TRP36 氨基酸序列的熵分析分别显示了 477 个和 388 个高熵峰。对 B 细胞表位分析显示，P28 和 TRP36 序列的保守氨基酸也得到了证明。因此，这些结果可用于提高对泰国埃立克体的系统发育关系、遗传多样性和抗原性的认识。
Zotero 插件