Panina Yulia S, Timechko Elena E, Usoltseva Anna A, Yakovleva Kristina D, Kantimirova Elena A, Dmitrenko Diana V
Department of Medical Genetics and Clinical Neurophysiology, Institute of Postgraduate Education, V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 660022 Krasnoyarsk, Russia.
Metabolites. 2023 Jan 4;13(1):83. doi: 10.3390/metabo13010083.
Temporal lobe epilepsy (TLE) is the most common type of focal epilepsy in adults. Experimental and clinical data indicate that neuroinflammation and neurodegeneration accompanying epileptogenesis make a significant contribution to the chronicity of epilepsy and the development of drug resistance in TLE cases. Changes in plasma and serum concentrations of proteins associated with neuroinflammation and neurodegeneration can be predictive biomarkers of the course of the disease. This study used an enzyme-linked immunosorbent assay of the following plasma proteins: brain-derived neurotrophic factor (), tumor necrosis factor alpha (), and high-mobility group protein B1 () in patients with mesial TLE to search for biomarkers of the disease. The objective of the study was to examine biomarkers of the neuroinflammation and neurodegeneration of plasma: , , and . The aim of the study was to identify changes in the concentration of circulating pro-inflammatory and neurotrophic factors that are prognostically significant for the development of drug resistance and the course of TLE. A decrease in the concentration of , , and was registered in the group of patients with TLE compared with the control group. A significant decrease in the concentration of HMGB1 in patients with drug-resistant TLE was observed. Aberrations in plasma concentrations of , , and in patients with TLE compared with the controls have been confirmed by earlier studies. A decrease in the expression of the three biomarkers may be the result of neurodegenerative processes caused by the long course of the disease. The results of the study may indicate the acceptability of using and as prognostic biological markers to indicate the severity of the disease course and the risk of developing drug resistance.
颞叶癫痫（TLE）是成人中最常见的局灶性癫痫类型。实验和临床数据表明，癫痫发生过程中伴随的神经炎症和神经退行性变对癫痫的慢性化以及TLE病例中耐药性的发展有重大影响。与神经炎症和神经退行性变相关的血浆和血清蛋白浓度变化可以作为疾病进程的预测生物标志物。本研究采用酶联免疫吸附测定法检测内侧颞叶癫痫患者血浆中的以下蛋白质：脑源性神经营养因子（）、肿瘤坏死因子α（）和高迁移率族蛋白B1（），以寻找该疾病的生物标志物。该研究的目的是检测血浆中神经炎症和神经退行性变的生物标志物：、和。该研究的目标是确定循环促炎因子和神经营养因子浓度的变化，这些变化对耐药性的发展和TLE的病程具有预后意义。与对照组相比，TLE患者组中、和的浓度降低。观察到耐药性TLE患者中HMGB1浓度显著降低。早期研究已证实，与对照组相比，TLE患者血浆中、和的浓度存在异常。这三种生物标志物表达的降低可能是疾病长期病程导致神经退行性变过程的结果。该研究结果可能表明，使用和作为预后生物标志物来指示疾病进程的严重程度和产生耐药性的风险是可行的。
