Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.
Res Vet Sci. 2023 Feb;155:126-136. doi: 10.1016/j.rvsc.2023.01.013. Epub 2023 Jan 18.
ALV-J-SD1005 strain was subcutaneously inoculated into the necks of 1-day-old HY-Line Brown chickens and caused severe growth retardation, viremia and subcutaneous fibrosarcomas in the necks of all infected chickens from 14 days post inoculation (DPI) to 21 DPI, and also significantly increased the expressions of TRIM25, P53, etc., but significantly decreased the expressions of 14-3-3σ, etc. Overexpression of chicken TRIM25 (chTRIM25) significantly promoted cell proliferation and improved the expressions of P53, CDC2, and CDK2 tumor factors; and significantly inhibited the expression of 14-3-3σ in ALV-J-SD1005-infected DF1 cells; but knockdown of chTRIM25 caused the opposite effects. The results of co-immunoprecipitation (Co-IP) and confocal microscopy confirmed that chTRIM25 can recognize and bind 14-3-3σ protein in ALV-J-SD1005-infected cells, and they were co-located in the cytoplasm. It can be concluded that chTRIM25 participates in the fibrous tissue hyperplasia induced by ALV-J-SD1005 infections in chickens by binding 14-3-3σ protein and regulating the expressions of 14-3-3σ, P53, CDC2, and CDK2.
ALV-J-SD1005 株皮下接种 1 日龄海兰褐雏鸡，可引起感染雏鸡从接种后 14 天至 21 天严重生长迟缓、血病毒血症和颈部皮下纤维肉瘤，且显著增加 TRIM25、P53 等的表达，但显著降低 14-3-3σ 等的表达。鸡 TRIM25（chTRIM25）过表达显著促进细胞增殖，提高 P53、CDC2 和 CDK2 肿瘤因子的表达，并显著抑制 ALV-J-SD1005 感染的 DF1 细胞中 14-3-3σ 的表达；而 chTRIM25 的敲低则产生相反的效果。免疫共沉淀（Co-IP）和共聚焦显微镜的结果证实，chTRIM25 可识别和结合 ALV-J-SD1005 感染细胞中的 14-3-3σ 蛋白，且它们在细胞质中共定位。由此可以得出结论，chTRIM25 通过结合 14-3-3σ 蛋白并调节 14-3-3σ、P53、CDC2 和 CDK2 的表达，参与了 ALV-J-SD1005 感染鸡引起的纤维组织增生。
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