使用铀石蜡反应对人表皮神经内分泌默克尔细胞进行染色。

Staining of neuroendocrine Merkel cells of human epidermis using the uranaffin reaction.

作者信息

Beiras A, García-Caballero T, Gallego R, Rosón E

机构信息

Departamento de Ciencias Morfológicas (Histología Humana), Facultad de Medicina, Hospital General de Galicia, Universidad de Santiago de Compostela, Spain.

出版信息

J Invest Dermatol. 1987 Oct;89(4):366-8. doi: 10.1111/1523-1747.ep12471756.

DOI:10.1111/1523-1747.ep12471756

PMID:3668279

Abstract

The uranaffin reaction (UR) stains neurosecretory (NS) granules of the neuroendocrine system under certain experimental conditions of staining and rinsing solutions. Human normal neuroendocrine (NE) Merkel cells stained using the UR exhibit a positive reaction in their NS granules, ribosomes, and nuclear chromatin. The average values of maximum granular diameter (GD = 69.1 nm) and area (GA = 3637.8 mm2) of NS granules measured in the adult NE Merkel cells stained with UR are significantly greater than those (GD = 61.4 nm; GA = 2699.8 nm2) seen in the fetal NE Merkel cells also stained with UR. No differences in form factor are found between fetal and adult NS granules. On different samples of human adult and fetal epidermis it is demonstrated that UR is a useful cytochemical marker for the NS granules of normal NE Merkel cells.

摘要

在特定的染色和冲洗溶液实验条件下，铀亲和素反应（UR）可对神经内分泌系统的神经分泌（NS）颗粒进行染色。使用UR染色的人类正常神经内分泌（NE）默克尔细胞在其NS颗粒、核糖体和核染色质中呈现阳性反应。在用UR染色的成年NE默克尔细胞中测量的NS颗粒最大颗粒直径（GD = 69.1 nm）和面积（GA = 3637.8 mm2）的平均值显著大于在用UR染色的胎儿NE默克尔细胞中观察到的数值（GD = 61.4 nm；GA = 2699.8 nm2）。胎儿和成年NS颗粒之间未发现形状因子差异。在人类成年和胎儿表皮的不同样本上证明，UR是正常NE默克尔细胞NS颗粒的一种有用的细胞化学标记物。