Tang Hua-Zhen, Yang Zhen-Peng, Lu Shuai, Wang Bing, Wang Yu-Ying, Sun Xi-Bo, Qu Jin-Xiu, Rao Ben-Qiang
Department of Gastrointestinal Surgery, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China.
Department of Gastrointestinal Surgery, Key Laboratory of Cancer FSMP for State Market Regulation, Beijing 100038, China.
World J Gastrointest Oncol. 2023 Jan 15;15(1):90-101. doi: 10.4251/wjgo.v15.i1.90.
BACKGROUND: Heat-clearing and detoxifying drugs has protective effect on colorectal cancer (CRC). Given the complicated features of Traditional Chinese medicine formulas, network pharmacology is an effective approach for studying the multiple interactions between drugs and diseases. AIM: To systematically explore the anticancer mechanism of heat-clearing and detoxifying drug JC724. METHODS: This study obtained the active compounds and their targets in JC724 from Traditional Chinese Medicine System Pharmacology Database. In addition, the CRC targets were obtained from Drugbank, TTD, DisGeNET and GeneCards databases. We performed transcriptome analysis of differentially expressed genes in CRC treated with JC724. Venn diagram was used to screen the JC724-CRC intersection targets as candidate targets. Core targets were selected by protein-protein interaction network and herb ingredient-target-disease network analysis. The functional and pathway of core targets were analysed by enrichment analysis. RESULTS: We found 174 active ingredients and 283 compound targets from JC724. 940 CRC-related targets were reserved from the four databases and 304 CRC differentially expressed genes were obtained by transcriptome analysis. We constructed the network and found that the five core ingredients were quercetin, β Beta sitosterol, wogonin, kaempferol and baicalein. The core JC724-CRC targets were , , , and . JC724 acts on multiple signaling pathways associated with CRC, including the Nrf2 signaling pathway, oxidative stress, and the IL-17 signaling pathway. CONCLUSION: In this study, we systematically analyzed the active ingredients, core targets and main mechanisms of JC724 in the treatment of CRC. This study could bring a new perspective to the heat-clearing and detoxifying therapy of CRC.
背景：清热解毒药物对结直肠癌（CRC）具有保护作用。鉴于中药方剂的复杂特性，网络药理学是研究药物与疾病之间多重相互作用的有效方法。 目的：系统探究清热解毒药物JC724的抗癌机制。 方法：本研究从中药系统药理学数据库中获取JC724中的活性成分及其靶点。此外，CRC靶点从DrugBank、TTD、DisGeNET和GeneCards数据库中获取。我们对用JC724处理的CRC中差异表达基因进行了转录组分析。使用维恩图筛选JC724-CRC交集靶点作为候选靶点。通过蛋白质-蛋白质相互作用网络和草药成分-靶点-疾病网络分析选择核心靶点。通过富集分析对核心靶点的功能和途径进行分析。 结果：我们从JC724中发现了174种活性成分和283个化合物靶点。从四个数据库中保留了940个与CRC相关的靶点，并通过转录组分析获得了304个CRC差异表达基因。我们构建了网络，发现五个核心成分是槲皮素、β-谷甾醇、汉黄芩素、山奈酚和黄芩苷。核心JC724-CRC靶点是……（此处原文缺失具体内容）。JC724作用于与CRC相关的多个信号通路，包括Nrf2信号通路、氧化应激和IL-17信号通路。 结论：在本研究中，我们系统分析了JC724治疗CRC的活性成分、核心靶点和主要机制。本研究可为CRC的清热解毒治疗带来新的视角。
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