Department of Biotechnology, Bilecik Şeyh Edebali University, Fatih Sultan Mehmet Bulvarı No:27, 11100 Bilecik Merkez/Bilecik, Turkey.
Department of Medical Services and Techniques, Bilecik Şeyh Edebali University, Vocational School of Health Services, Fatih Sultan Mehmet Bulvarı No:27, 11100 Bilecik Merkez/Bilecik, Turkey.
Lett Appl Microbiol. 2023 Jan 23;76(1). doi: 10.1093/lambio/ovac034.
The functional food ingredients market has been growing due to the preferences for healthier, nutritional, environment-friendly, and convenience foods. Here, we evaluated the antimicrobial potential of the lyophilized cell-free supernatants of the two most promising oral probiotic strains Streptococcus salivarius M18 and S. salivarius K12 on Pseudomonas aeruginosa to be applied for safety purposes in the milk. We showed that the lyophilized culture supernatant of the strain M18 inhibited the pathogen growth in milk by about 75%, 70%, and 60% when incubated at 37°C, room temperature, and +4°C, respectively. The inhibition levels were about 50%, 30%, and 45% for the lyophilized K12 cell-free supernatant. Besides, the lyophilized culture supernatants of the oral probiotics, especially of S. salivarius M18, exhibited anti-cancer activities on colon cancer cells in vitro. Thus, the results of this manuscript suggest that the cell-free supernatants of the M18 and K12 strains are potential candidates, which merit more investigation for their applications, as biopreservatives in foods and beverages and as anti-cancer biotics for human health.
由于人们对健康、营养、环保和方便食品的偏好，功能性食品成分市场一直在增长。在这里，我们评估了两种最有前途的口腔益生菌菌株——唾液链球菌 M18 和 S. salivarius K12 的冻干无细胞上清液对铜绿假单胞菌的抗菌潜力，以便将其安全应用于牛奶中。我们表明，当在 37°C、室温（20-25°C）和+4°C 下孵育时，菌株 M18 的冻干培养上清液分别使牛奶中的病原体生长抑制了约 75%、70%和 60%。对于冻干的 K12 无细胞上清液，抑制水平约为 50%、30%和 45%。此外，口腔益生菌的冻干培养上清液，特别是唾液链球菌 M18 的上清液，在体外对结肠癌细胞表现出抗癌活性。因此，本文的结果表明，M18 和 K12 菌株的无细胞上清液是有潜力的候选物，值得进一步研究其在食品和饮料中的生物防腐剂以及作为人类健康的抗癌抗生素的应用。
