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与土壤传播线虫相关的细菌的昆虫病原潜力和昆虫对其感染的免疫反应。

Entomopathogenic potential of bacteria associated with soil-borne nematodes and insect immune responses to their infection.

机构信息

Department of Plant Health and Environment, Laboratory of Bio-Aggressor and Integrated Protection in Agriculture, National Agronomic Institute of Tunisia, University of Carthage, Tunis, Tunisia.

Department of Theoretical and Applied Sciences, Laboratory of Comparative Immunology and Parasitology, University of Insubria, Varese, Italy.

出版信息

PLoS One. 2023 Jan 23;18(1):e0280675. doi: 10.1371/journal.pone.0280675. eCollection 2023.

Abstract

Soil-borne nematodes establish close associations with several bacterial species. Whether they confer benefits to their hosts has been investigated in only a few nematode-bacteria systems. Their ecological function, therefore, remains poorly understood. In this study, we isolated several bacterial species from rhabditid nematodes, molecularly identified them, evaluated their entomopathogenic potential on Galleria mellonella larvae, and measured immune responses of G. mellonella larvae to their infection. Bacteria were isolated from Acrobeloides sp., A. bodenheimeri, Heterorhabditis bacteriophora, Oscheius tipulae, and Pristionchus maupasi nematodes. They were identified as Acinetobacter sp., Alcaligenes sp., Bacillus cereus, Enterobacter sp., Kaistia sp., Lysinibacillus fusiformis, Morganella morganii subsp. morganii, Klebsiella quasipneumoniae subsp. quasipneumoniae, and Pseudomonas aeruginosa. All bacterial strains were found to be highly entomopathogenic as they killed at least 53.33% G. mellonella larvae within 72h post-infection, at a dose of 106 CFU/larvae. Among them, Lysinibacillus fusiformis, Enterobacter sp., Acinetobacter sp., and K. quasipneumoniae subsp. quasipneumoniae were the most entomopathogenic bacteria. Insects strongly responded to bacterial infection. However, their responses were apparently little effective to counteract bacterial infection. Our study, therefore, shows that bacteria associated with soil-borne nematodes have entomopathogenic capacities. From an applied perspective, our study motivates more research to determine the potential of these bacterial strains as biocontrol agents in environmentally friendly and sustainable agriculture.

摘要

土壤线虫与几种细菌建立密切的联系。它们是否对其宿主有益,仅在少数线虫-细菌系统中进行了研究。因此,它们的生态功能仍知之甚少。在这项研究中,我们从小杆线虫中分离出几种细菌,对其进行分子鉴定,评估它们对黄粉虫幼虫的昆虫病原潜力,并测量黄粉虫幼虫对其感染的免疫反应。细菌是从小杆线虫属、A. bodenheimeri、嗜线虫杆菌、Oscheius tipulae 和 Pristionchus maupasi 中分离出来的。它们被鉴定为不动杆菌属、产碱杆菌属、蜡样芽孢杆菌、肠杆菌属、卡氏菌属、溶杆菌属、摩根菌属亚种摩根菌、肺炎克雷伯菌亚种肺炎克雷伯菌和铜绿假单胞菌。所有细菌菌株都被发现具有高度的昆虫病原性,因为它们在感染后 72 小时内以 106 CFU/幼虫的剂量至少杀死了 53.33%的黄粉虫幼虫。其中,溶杆菌属、肠杆菌属、不动杆菌属和肺炎克雷伯菌亚种肺炎克雷伯菌是最具昆虫病原性的细菌。昆虫对细菌感染强烈反应。然而,它们的反应显然对抵抗细菌感染没有什么效果。因此,我们的研究表明,与土壤线虫相关的细菌具有昆虫病原性。从应用的角度来看,我们的研究促使更多的研究确定这些细菌菌株作为环保和可持续农业中生物防治剂的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/121f/10045567/fe020cece804/pone.0280675.g001.jpg

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