定位东乡普通野生稻（Oryza rufipogon Griff.）中与愈伤组织褐化相关的数量性状基因座。

Mapping quantitative trait loci associated with callus browning in Dongxiang common wild rice (Oryza rufipogon Griff.).

作者信息

Wang Yibo, Yang Xin, Xu Guimin, Ye Xiao, Ji Yiming, Lou Xin, Su Jingjing, Sun Chuanqing, Fu Yongcai, Zhang Kun

机构信息

MOE Key Laboratory of Crop Heterosis and Utilization, National Center for Evaluation of Agricultural Wild Plants (Rice), Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and Breeding, China Agricultural University, Beijing, 100193, China.

Rural Development Institute, Chinese Academy of Social Sciences, Beijing, 100073, China.

出版信息

Mol Biol Rep. 2023 Apr;50(4):3129-3140. doi: 10.1007/s11033-023-08279-7. Epub 2023 Jan 24.

DOI:10.1007/s11033-023-08279-7

PMID:36692673

Abstract

BACKGROUND

Genetic transformation of indica rice (Oryza sativa ssp. indica) is limited by callus browning, which results in poor in vitro tissue culturability. Elucidating the genes in common wild rice (Oryza rufipogon Griff.) that control callus browning is fundamental for improving the tissue culturability of indica rice varieties.

METHODS AND RESULTS

We used a population of 129 O. rufipogon (Dongxiang common wild rice; DXCWR) introgression lines in the elite cultivar GC2 (Oryza sativa ssp. indica) background and 159 simple sequence repeat (SSR) markers to identify quantitative trait loci (QTLs) associated with callus browning. We evaluated callus browning based on the indices of callus browning rate (CBR), callus browning index (CBI), and standard callus browning index (SCBI).

CONCLUSIONS

We detected 30 QTLs associated with callus browning across all lines, mapping to chromosomes 1, 2, 3, 4, 8, 9, and 12. These genomic regions were repeatedly associated with differences in CBR, CBI, and SCBI. The alleles from DXCWR showed additive effects in reducing callus browning. We identified new QTLs near the markers RM247 and RM7003 on chromosome 12, indicating that these QTLs are unique to DXCWR. Furthermore, we identified six introgression lines with significantly lower callus browning. These lines will be useful germplasms for genetic transformation and fine-mapping of the culturability trait.

摘要

背景

籼稻（Oryza sativa ssp. indica）的遗传转化受到愈伤组织褐化的限制，这导致其体外组织培养能力较差。阐明普通野生稻（Oryza rufipogon Griff.）中控制愈伤组织褐化的基因是提高籼稻品种组织培养能力的基础。

方法与结果

我们利用129个东乡普通野生稻（DXCWR）渗入系群体，其遗传背景为优良品种GC2（Oryza sativa ssp. indica），并使用159个简单序列重复（SSR）标记来鉴定与愈伤组织褐化相关的数量性状位点（QTL）。我们基于愈伤组织褐化率（CBR）、愈伤组织褐化指数（CBI）和标准愈伤组织褐化指数（SCBI）等指标评估愈伤组织褐化情况。

结论

我们在所有品系中检测到30个与愈伤组织褐化相关的QTL，这些QTL定位于第1、2、3、4、8、9和12号染色体上。这些基因组区域反复与CBR、CBI和SCBI的差异相关。来自DXCWR的等位基因在降低愈伤组织褐化方面表现出加性效应。我们在第12号染色体上的标记RM247和RM7003附近鉴定到新的QTL，表明这些QTL是DXCWR特有的。此外，我们鉴定出六个愈伤组织褐化显著较低的渗入系。这些品系将是用于遗传转化和培养能力性状精细定位的有用种质。