Kubota Masaaki, Zhang Bo-Shi, Li Shu-Yang, Yoshida Yoichi, Wang Hao, Adachi Akihiko, Matsutani Tomoo, Mine Seiichiro, Machida Toshio, Kamitsukasa Ikuo, Wada Takeshi, Aotsuka Akiyo, Kitamura Kenichiro, Takizawa Hirotaka, Kuroda Hideyuki, Iwadate Yasuo, Hiwasa Takaki
Department of Neurological Surgery, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan.
Comprehensive Stroke Center, Chiba University Hospital, Chiba 260-8677, Japan.
Med Int (Lond). 2022 Dec 21;3(1):4. doi: 10.3892/mi.2022.64. eCollection 2023 Jan-Feb.
Autoantibodies can be used in the early diagnosis and treatment of atherosclerosis-related diseases. Using ProtoArray screening of samples from patients with atherosclerosis, the present study identified thiosulfate sulfurtransferase-like domain-containing 2 (TSTD2) as a novel atherosclerosis antigen. The serum TSTD2 antibody levels were then quantified using an amplified luminescent proximity homogeneous assay-linked immunosorbent assay. This demonstrated the levels of TSTD2 antibodies (TSTD2-Abs) to be significantly higher in patients with acute cerebral infarction or chronic kidney disease than in healthy donors. The TSTD2-Ab levels were also found to be higher in males, older adults, smokers, in those who consumed alcohol regularly, and in those with hypertension. Furthermore, Spearman's rank correlation analysis revealed TSTD2-Ab levels to be strongly associated with measures of atherosclerosis severity, including plaque scores, intima-media thickness of the carotid artery and the cardio-ankle vascular index. Thus, TSTD2-Abs may thus be a promising novel biomarker for atherosclerosis-related cerebral infarction and kidney disease.
自身抗体可用于动脉粥样硬化相关疾病的早期诊断和治疗。本研究通过对动脉粥样硬化患者样本进行ProtoArray筛选，鉴定出含硫代硫酸盐硫转移酶样结构域2（TSTD2）为一种新型动脉粥样硬化抗原。然后使用放大发光邻近均相分析联免疫吸附测定法定量血清TSTD2抗体水平。结果表明，急性脑梗死或慢性肾病患者的TSTD2抗体（TSTD2-Abs）水平显著高于健康供体。还发现男性、老年人、吸烟者、经常饮酒者以及高血压患者的TSTD2-Ab水平更高。此外，Spearman等级相关分析显示，TSTD2-Ab水平与动脉粥样硬化严重程度指标密切相关，包括斑块评分、颈动脉内膜中层厚度和心踝血管指数。因此，TSTD2-Abs可能是动脉粥样硬化相关脑梗死和肾病的一种有前景的新型生物标志物。
