Waglewska Ewelina, Maliszewska Irena, Bazylińska Urszula
Department of Physical and Quantum Chemistry, Faculty of Chemistry, Wroclaw University of Science and Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland.
Department of Organic and Medical Chemistry, Faculty of Chemistry, Wroclaw University of Science and Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland.
J Photochem Photobiol B. 2023 Mar;240:112650. doi: 10.1016/j.jphotobiol.2023.112650. Epub 2023 Jan 14.
Widespread skin infections caused primarily by bacteria and yeast, pose a growing threat to healthcare systems. Phyto-photodynamic antimicrobial therapy is a promising treatment strategy with a few mild side effects for both superficial and deeper skin infections. The combination of natural plant products (polyphenols) with conventional photosensitizers makes it possible to improve the outcome of skin infections. In the present study, nanoengineered self-assembling bilosomes were used as a nanoplatform to deliver two compounds with different solubility, i.e., curcumin applied as a hydrophobic phytochemical compound and Methylene Blue used as a hydrophilic photosensitizer. Compared with the encapsulation of Methylene Blue alone, the double-loaded bilosomes (curcumin-supported Methylene Blue) showed higher efficiency in generating reactive oxygen species. Importantly, in our study, we also confirmed that bioinspired bilosomes prevent the rapid photobleaching of Methylene Blue, thereby enhancing its photoactivity. The post-irradiation antimicrobial action was tested against two pathogens - the Gram-positive bacterium (Staphylococcus aureus) and yeast (Candida albicans). The irradiation was provided after 10, 20, and 30 min, at a specific wavelength (λ = 640 nm) corresponding to 63, 126, and 189 J cm energy fluences. The most effective reduction in the microbial cells number was found 30 min post-irradiation and was 99.994% for double-loaded bilosomes compared to 99.989% killing S. aureus for bilosomes with Methylene Blue alone. For C. albicans fungal cells, the mortality was 99.669% in the presence of a Methylene Blue and curcumin mixture compared to 98.229% of those killed without the addition of curcumin. The overall results of our contribution provide evidence that curcumin in combination with MB enhances the photo-eradication efficiency of S. aureus and C. albicans planktonic cultures. Thus, the mixture of the phytochemicals with photosensitizers and their encapsulation in multifunctional bilosomes may contribute to the development of innovative antimicrobial phyto-photodynamic therapy in the future.
主要由细菌和酵母菌引起的广泛皮肤感染，对医疗系统构成了日益严重的威胁。植物光动力抗菌疗法是一种很有前景的治疗策略，对浅表和深层皮肤感染都有一些轻微的副作用。天然植物产品（多酚）与传统光敏剂的结合使得改善皮肤感染的治疗效果成为可能。在本研究中，纳米工程自组装双分子层脂质体被用作纳米平台，用于递送两种具有不同溶解度的化合物，即作为疏水性植物化学化合物应用的姜黄素和作为亲水性光敏剂使用的亚甲蓝。与单独包封亚甲蓝相比，双负载双分子层脂质体（姜黄素负载的亚甲蓝）在产生活性氧方面表现出更高的效率。重要的是，在我们的研究中，我们还证实了仿生双分子层脂质体可防止亚甲蓝的快速光漂白，从而增强其光活性。对两种病原体——革兰氏阳性菌（金黄色葡萄球菌）和酵母菌（白色念珠菌）进行了辐照后抗菌作用测试。在10、20和30分钟后，在对应于63、126和189 J/cm能量通量的特定波长（λ = 640 nm）下进行辐照。辐照后30分钟发现微生物细胞数量减少最为有效，双负载双分子层脂质体的减少率为99.994%，而单独含有亚甲蓝的双分子层脂质体对金黄色葡萄球菌的杀灭率为99.989%。对于白色念珠菌真菌细胞，在存在亚甲蓝和姜黄素混合物的情况下死亡率为99.669%，而在不添加姜黄素的情况下死亡率为98.229%。我们研究的总体结果提供了证据，表明姜黄素与亚甲蓝联合可提高金黄色葡萄球菌和白色念珠菌浮游培养物的光根除效率。因此，植物化学物质与光敏剂的混合物及其在多功能双分子层脂质体中的包封可能有助于未来创新抗菌植物光动力疗法的发展。
