一种改进的从海岸碎屑沉积物中制备花粉样本的程序。
An improved preparation procedure for pollen samples from coastal clastic sediments.
作者信息
Yao Qiang, Liu Kam-Biu, Rodrigues Erika
机构信息
Department of Oceanography and Coastal Sciences and Coastal Studies Institute, Louisiana State University, Baton Rouge, LA 70803, USA.
Institute of Geosciences, University of São Paulo, São Paulo, Brazil.
出版信息
MethodsX. 2023 Jan 13;10:102016. doi: 10.1016/j.mex.2023.102016. eCollection 2023.
Palynological analysis is a time-tested analytical method in the field of geology, geography, and archaeology across the globe. However, a major problem in palynology is that due to the dynamic nature of coastal and lake settings, sediment samples from these environments usually contain large proportion of clastic materials that are difficult to remove and isolate from pollen grains. In this study, we present a step-by-step procedure of an optimized preparation method to eliminate the organic and clastic materials and concentrate the pollen grains. We also present some insights on how to prepare a clean microscopic slide with intact pollen grains. We believe this procedure can successfully eliminate organic and clastic materials and concentrate the pollen grains to produce an ideal microscopic slide for pollen analysis.•Extract samples and eliminate carbonate materials with hydrochloric acid.•Remove organic materials with potassium hydroxide.•Remove clastic materials with sieving and hydrofluoric acid.
孢粉学分析是全球地质、地理和考古领域经过时间检验的分析方法。然而，孢粉学中的一个主要问题是，由于沿海和湖泊环境的动态性质，这些环境中的沉积物样本通常含有很大比例的碎屑物质，这些物质很难从花粉粒中去除和分离。在本研究中，我们提出了一种优化制备方法的分步程序，以去除有机和碎屑物质并浓缩花粉粒。我们还就如何制备带有完整花粉粒的干净载玻片提出了一些见解。我们相信该程序能够成功去除有机和碎屑物质并浓缩花粉粒，从而制作出用于花粉分析的理想载玻片。
•提取样本并用盐酸去除碳酸盐物质。
•用氢氧化钾去除有机物质。
•通过筛分和氢氟酸去除碎屑物质。
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