Faculty of Life Science and Technology, Kunming University of Science and Technology, 650500, Kunming, China.
UWA School of Agriculture and Environment, The University of Western Australia, 35 Stirling Highway, Perth, WA, 6009, Australia; Institute for Adriatic Crops and Karst Reclamation, 21000 Split, Croatia.
J Plant Physiol. 2023 Mar;282:153917. doi: 10.1016/j.jplph.2023.153917. Epub 2023 Jan 17.
Reactive oxygen species (ROS) and nitric oxide (NO) are important signaling molecules regulating stomatal movements in plants. Melatonin (N-acetyl-5-methoxytryptamine) was found to induce stomatal closure via phytomelatonin receptor 1 (PMTR1)-mediated activation of ROS production. Here, we evaluated the interaction between ROS and NO in the melatonin-induced stomatal closure in Arabidopsis. The results showed that the exogenous melatonin-induced stomatal closure and NO production were abolished by carboxy-PTIO (cPTIO, a NO scavenger). Additionally, the mutant lines nitrate reductase 1 and 2 (nia1nia2) and NO-associated 1 (noa1) did not show melatonin-induced stomatal closure, indicating that the melatonin-mediated stomatal closure is dependent on NO. The application of HO induced the NO production and stomatal closure in the presence or absence of melatonin. However, the melatonin-induced NO production was impaired in the rhohC and rbohD/F (NADPH oxidase respiratory burst oxidase homologs) mutant plants. Furthermore, the ROS levels in nia1nia2 and noa1 did not differ significantly from the wild type plants, indicating that NO is a downstream component in the melatonin-induced ROS production. Exogenous melatonin did not induce NO and ROS production in the guard cells of pmtr1 mutant lines, suggesting NO occurs downstream of ROS in the PMTR1-mediated stomatal closure in Arabidopsis. Taken together, the results presented here suggest that melatonin-induced stomatal closure via PMTR1-mediated signaling in the regulation of ROS and NO production in Arabidopsis.
活性氧 (ROS) 和一氧化氮 (NO) 是调节植物气孔运动的重要信号分子。褪黑素 (N-乙酰-5-甲氧基色胺) 被发现通过植物褪黑素受体 1 (PMTR1) 介导的 ROS 产生的激活诱导气孔关闭。在这里，我们评估了 ROS 和 NO 在褪黑素诱导的拟南芥气孔关闭中的相互作用。结果表明，外源褪黑素诱导的气孔关闭和 NO 产生被羧基-PTIO (cPTIO，一种 NO 清除剂) 所消除。此外，硝酸还原酶 1 和 2 (nia1nia2) 和与 NO 相关的 1 (noa1) 突变体没有表现出褪黑素诱导的气孔关闭，表明褪黑素介导的气孔关闭依赖于 NO。HO 的应用在有或没有褪黑素的情况下诱导 NO 产生和气孔关闭。然而，在 rhohC 和 rbohD/F (NADPH 氧化酶呼吸爆发氧化酶同源物) 突变体植物中，褪黑素诱导的 NO 产生受损。此外，nia1nia2 和 noa1 中的 ROS 水平与野生型植物没有显著差异，表明 NO 是褪黑素诱导的 ROS 产生的下游成分。外源褪黑素在 pmtr1 突变体系的保卫细胞中没有诱导 NO 和 ROS 产生，表明在 PMTR1 介导的拟南芥气孔关闭中，NO 发生在 ROS 的下游。总之，这些结果表明，褪黑素通过 PMTR1 介导的信号转导诱导拟南芥中 ROS 和 NO 产生，从而诱导气孔关闭。
