解析花生属物种中 WRKY 基因的进化动态。

Deciphering evolutionary dynamics of WRKY genes in Arachis species.

机构信息

Key Laboratory of National Forestry and Grassland Administration On Grassland Resources and Ecology in the Yellow River Delta, College of Grassland Science, Qingdao Agricultural University, Qingdao, China.

Qingdao Key Laboratory of Specialty Plant Germplasm Innovation and Utilization in Saline Soils of Coastal Beach, College of Grassland Science, Qingdao Agricultural University, Qingdao, China.

出版信息

BMC Genomics. 2023 Jan 27;24(1):48. doi: 10.1186/s12864-023-09149-z.

DOI:10.1186/s12864-023-09149-z

PMID:36707767

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9881300/

Abstract

BACKGROUND

Cultivated peanut (Arachis hypogaea), a progeny of the cross between A. duranensis and A. ipaensis, is an important oil and protein crop from South America. To date, at least six Arachis genomes have been sequenced. WRKY transcription factors (TFs) play crucial roles in plant growth, development, and response to abiotic and biotic stresses. WRKY TFs have been identified in A. duranensis, A. ipaensis, and A. hypogaea cv. Tifrunner; however, variations in their number and evolutionary patterns across various Arachis spp. remain unclear.

RESULTS

WRKY TFs were identified and compared across different Arachis species, including A. duranensis, A. ipaensis, A. monticola, A. hypogaea cultivars (cv.) Fuhuasheng, A. hypogaea cv. Shitouqi, and A. hypogaea cv. Tifrunner. The results showed that the WRKY TFs underwent dynamic equilibrium between diploid and tetraploid peanut species, characterized by the loss of old WRKY TFs and retention of the new ones. Notably, cultivated peanuts inherited more conserved WRKY orthologs from wild tetraploid peanuts than their wild diploid donors. Analysis of the W-box elements and protein-protein interactions revealed that different domestication processes affected WRKY evolution across cultivated peanut varieties. WRKY TFs of A. hypogaea cv. Fuhuasheng and Shitouqi exhibited a similar domestication process, while those of cv. Tifrunner of the same species underwent a different domestication process based on protein-protein interaction analysis.

CONCLUSIONS

This study provides new insights into the evolution of WRKY TFs in Arachis spp.

摘要

背景

栽培花生（Arachis hypogaea）是 A. duranensis 和 A. ipaensis 杂交的后代，是一种来自南美洲的重要油料和蛋白质作物。迄今为止，至少有六个 Arachis 基因组已被测序。WRKY 转录因子（TFs）在植物的生长、发育以及对非生物和生物胁迫的响应中起着至关重要的作用。WRKY TFs 已在 A. duranensis、A. ipaensis 和 A. hypogaea cv. Tifrunner 中被鉴定；然而，它们在不同 Arachis 种中的数量和进化模式的变化仍不清楚。

结果

在不同的 Arachis 物种中鉴定和比较了 WRKY TFs，包括 A. duranensis、A. ipaensis、A. monticola、A. hypogaea 品种（cv.）Fuhuasheng、A. hypogaea cv. Shitouqi 和 A. hypogaea cv. Tifrunner。结果表明，WRKY TFs 在二倍体和四倍体花生物种之间经历了动态平衡，表现为旧 WRKY TFs 的丢失和新 WRKY TFs 的保留。值得注意的是，栽培花生从野生四倍体花生中继承了更多保守的 WRKY 直系同源物，而不是它们的野生二倍体供体。W-盒元件和蛋白质-蛋白质相互作用的分析表明，不同的驯化过程影响了栽培花生品种中 WRKY 的进化。A. hypogaea cv. Fuhuasheng 和 Shitouqi 的 WRKY TFs 表现出相似的驯化过程，而同一物种 cv. Tifrunner 的 WRKY TFs 则根据蛋白质-蛋白质相互作用分析经历了不同的驯化过程。