具有良好水溶性的基于荧光共振能量转移的荧光探针，用于同时检测SO衍生物和粘度。
FRET-based fluorescent probe with favorable water solubility for simultaneous detection of SO derivatives and viscosity.
作者信息
Liu Tian-Zhen, Wang Shuo, Xu Jia-Rui, Miao Jun-Ying, Zhao Bao-Xiang, Lin Zhao-Min
机构信息
Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, PR China.
Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Qingdao, 266237, PR China.
出版信息
Talanta. 2023 May 1;256:124302. doi: 10.1016/j.talanta.2023.124302. Epub 2023 Jan 24.
The intracellular viscosity is an important parameter of the microenvironment and SO is a vital gas signal molecule. At present, some dual-response fluorescence probes for simultaneous measurements of viscosity and SO derivatives (HSO/SO) possessed poor water solubility. In this work, we developed a water-soluble fluorescence probe CIJ (0.0864 g/100 mL of water at 20 °C) for simultaneous measurements of viscosity and SO derivatives. CIJ exhibited a sensitive fluorescence enhancement to environmental viscosity from 0.97 to 28.04 cP based on a twisted intramolecular charge transfer mechanism and was applied to effective measurement of viscosity in vitro and in vivo. CIJ could also respond to SO derivatives with a low detection limit (44 nM) and a fast response time (5 min) based on the nucleophilic addition reaction. Furthermore, CIJ was applied to monitor SO derivatives in ratiometric response manner in living cells.
细胞内粘度是微环境的一个重要参数，而二氧化硫（SO）是一种重要的气体信号分子。目前，一些用于同时测量粘度和SO衍生物（亚硫酸氢根/二氧化硫，HSO⁻/SO₂）的双响应荧光探针水溶性较差。在这项工作中，我们开发了一种水溶性荧光探针CIJ（20℃时在水中的溶解度为0.0864 g/100 mL），用于同时测量粘度和SO衍生物。基于扭曲分子内电荷转移机制，CIJ对环境粘度从0.97到28.04 cP表现出灵敏的荧光增强，并应用于体外和体内粘度的有效测量。基于亲核加成反应，CIJ对SO衍生物也有响应，检测限低（44 nM），响应时间快（5分钟）。此外，CIJ还被应用于以比率响应方式监测活细胞中的SO衍生物。
Zotero 插件