暴露于环境和化学应激的大鼠体内放射性标记核蛋白的周转情况。
The turnover of radiolabeled nuclear proteins in rats exposed to environmental and chemical stress.
作者信息
Pipkin J L, Anson J F, Hinson W G, Duffy P H, Burns E R, Casciano D A
机构信息
Department of Health and Human Services, Food and Drug Administration, Jefferson, AR 72079.
出版信息
Toxicol Lett. 1987 Nov;39(1):15-26. doi: 10.1016/0378-4274(87)90252-9.
Exposure to a 12 h light/12 h dark (L/D) cycle for 1 month, followed by reversal to a 12 h D/12 h L (D/L) cycle stimulated within 18 h the incorporation of [3H]leucine and [32P]orthophosphoric acid into new proteins (130-25 kDa) in the G0 phase of the cell cycle of the non-regenerating and regenerating rat liver as observed in two-dimensional gel autoradiograms. Six additional proteins from the rat submaxillary gland (130-20 kDa) revealed labeling with 32P within 3 h following combined administration of isoproterenol and sodium arsenite. Labeling disappeared within 7 days for all stressed proteins.
将大鼠置于12小时光照/12小时黑暗（L/D）周期下1个月，然后反转至12小时黑暗/12小时光照（D/L）周期，在18小时内刺激未再生和再生大鼠肝脏细胞周期G0期新蛋白质（130 - 25 kDa）中[3H]亮氨酸和[32P]正磷酸的掺入，这在二维凝胶放射自显影片中可见。在联合给予异丙肾上腺素和亚砷酸钠后3小时内，大鼠颌下腺的另外六种蛋白质（130 - 20 kDa）显示出32P标记。所有应激蛋白质的标记在7天内消失。
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