Sun Yufan, Ren Tiantian, Zhao Jiateng, Zhao Wensheng, Nie Lanchun
College of Horticulture, Hebei Agricultural University, Baoding, 071000, Hebei, China.
College of Horticulture, Hebei Agricultural University, Baoding, 071000, Hebei, China; Hebei Key Laboratory of Vegetable Germplasm Innovation and Utilization, Baoding, 071000, Hebei, China; Collaborative Innovation Center of Vegetative Industry of Hebei Province, Baoding, 071000, Hebei, China.
Gene Expr Patterns. 2023 Mar;47:119306. doi: 10.1016/j.gep.2023.119306. Epub 2023 Feb 3.
In production, most cultivars of melon are andromonoecious and characterized by carrying both male and bisexual flowers on the same plant. In this study, four A-class genes (CmAP1a, CmAP1b, CmAP2a and CmAP2b), two B-class genes (CmAP3 and CmPI), two C-class genes (CmAGa and CmAGb) and four E-class genes (CmSEP1,2,3,4) were identified in melon. However, no D-class gene of melon was identified. The conserved domains of ABCE function proteins showed relatively high similarity between Arabidopsis and melon. The expression patterns of ABCE homeotic genes in different flower buds of melon suggested that transcripts of CmAP1a, CmPI and CmSEP1 in bisexual buds were significantly lower than that in male flower buds, while the expression levels of CmAGa, CmAGb and CmSEP4 in bisexual flower buds were significantly higher than that in male flower buds. There was no significant difference in expression levels of other ABCE model genes between male buds and bisexual buds. Subsequently, qRT-PCR was performed in different floral organs of bisexual flowers in melon. For A class genes, CmAP1a and CmAP1b showed the highest accumulation in sepals than petals, stamens and pistil, while CmAP2a and CmAP2b revealed the highest expression in pistil than other three floral organs. For B class genes, CmAP3 and CmPI were highly accumulated in petals and stamens though CmAP3 also showed abundant accumulation in pistil. For C class genes, the expression levels of CmAGa and CmAGb were higher in stamens and pistil than that in sepals and petals. For E class genes, CmSEP1 showed higher expression level in sepals and petals than stamens and pistil. CmSEP2, CmSEP3 and CmSEP4 showed the highest accumulation in pistil than other floral organs. These results provided a theoretical basis for studying the function of ABCE homeotic genes in floral organs development of melon.
在生产中，大多数甜瓜品种是雄全同株的，其特征是在同一植株上同时着生雄花和两性花。在本研究中，在甜瓜中鉴定出4个A类基因（CmAP1a、CmAP1b、CmAP2a和CmAP2b）、2个B类基因（CmAP3和CmPI）、2个C类基因（CmAGa和CmAGb）和4个E类基因（CmSEP1、2、3、4）。然而，未鉴定出甜瓜的D类基因。ABCE功能蛋白的保守结构域在拟南芥和甜瓜之间显示出相对较高的相似性。甜瓜不同花芽中ABCE同源异型基因的表达模式表明，两性花花芽中CmAP1a、CmPI和CmSEP1的转录本显著低于雄花花芽，而两性花花芽中CmAGa、CmAGb和CmSEP4的表达水平显著高于雄花花芽。雄花芽和两性花花芽中其他ABCE模型基因的表达水平没有显著差异。随后，对甜瓜两性花的不同花器官进行了qRT-PCR。对于A类基因，CmAP1a和CmAP1b在萼片中的积累量高于花瓣、雄蕊和雌蕊，而CmAP2a和CmAP2b在雌蕊中的表达量高于其他三个花器官。对于B类基因，CmAP3和CmPI在花瓣和雄蕊中高度积累，尽管CmAP3在雌蕊中也有大量积累。对于C类基因，CmAGa和CmAGb在雄蕊和雌蕊中的表达水平高于萼片和花瓣。对于E类基因，CmSEP1在萼片和花瓣中的表达水平高于雄蕊和雌蕊。CmSEP2、CmSEP3和CmSEP4在雌蕊中的积累量高于其他花器官。这些结果为研究ABCE同源异型基因在甜瓜花器官发育中的功能提供了理论依据。
