Michiels P, Delputte N, Debyser W, Langenaeken N A, Courtin C M
KU Leuven, Dept. Microbial and Molecular Systems, Laboratory of Food Chemistry and Biochemistry and Leuven Food Science and Nutrition Research Centre (LFoRCe), Kasteelpark Arenberg 20, box 2463, B-3000 Leuven, Belgium.
Anheuser-Busch InBev, Brouwerijplein 1, B-3000 Leuven, Belgium.
Carbohydr Polym. 2023 Apr 15;306:120597. doi: 10.1016/j.carbpol.2023.120597. Epub 2023 Jan 19.
The impact of arabinoxylan (AX) on the brewing process and beer characteristics depends on its content and structure and is often overlooked in research and industry. This paper reports on the occurrence and structural heterogeneity of AX in a set of commercial pilsner beers and their non-alcoholic counterparts. Fractionation by graded ethanol precipitation allowed us to isolate AX-rich fractions from beer with a number-average degree of polymerisation of 4 to 308 and an average degree of substitution in the range of 0.43 to 0.88. Pilsner beers had a higher content of high-molecular-weight AX than their non-alcoholic counterparts. The structural heterogeneity among the various commercial beers differed. By comparing the chemical composition of the beers, differences in beer production methods and ingredient selection were deduced and used to tentatively explain the differences in AX content and structural heterogeneity.
阿拉伯木聚糖(AX)对酿造过程和啤酒特性的影响取决于其含量和结构,而在研究和工业中这一点常常被忽视。本文报道了一组商业比尔森啤酒及其无醇啤酒对应产品中AX的存在情况和结构异质性。通过分级乙醇沉淀进行分离,使我们能够从啤酒中分离出富含AX的级分,其数均聚合度为4至308,平均取代度在0.43至0.88范围内。比尔森啤酒中高分子量AX的含量高于其无醇啤酒对应产品。不同商业啤酒之间的结构异质性有所不同。通过比较啤酒的化学成分,推断出啤酒生产方法和原料选择的差异,并用于初步解释AX含量和结构异质性的差异。