School of Chemistry and Chemical Engineering, Shandong Provincial Key Laboratory of Chemical Energy Storage and Novel Cell Technology, Liaocheng University, Liaocheng, 252059, China.
State Key Laboratory for Performance and Structure Safety of Petroleum Tubular Goods and Equipment Materials, Tubular Goods Research Institute, Xi'an, 710077, China.
Mikrochim Acta. 2023 Feb 7;190(3):82. doi: 10.1007/s00604-023-05665-7.
Novel and portable cotton swab-based fluorometry was constructed for the first time for 3-aminosalicylic acid (3-ASA) and 5-aminosalicylic acid (5-ASA) detection. It was carried out by fluorescence enhancement on silver (Ag)-doped black phosphorus quantum dots (Ag@BPQD). Ag@BPQD were prepared from AgNO and bulk black phosphorus in N, N-dimethylformamide (DMF) solution by solvothermal decomposition after mechanical exfoliation. Ag@BPQD show blue fluorescence with a quantum yield (QY) of 2.43%. In the presence of Ag@BPQD, 3-ASA exhibited bright blue fluorescence (λ = 328 nm, λ = 448 nm). The fluorescence of 5-ASA was also enhanced significantly and exhibited bright green emission (λ = 328 nm, λ = 484 nm). The linear range of 3-ASA is 0-90 μM with a detection limit (LOD) of 0.10 μM, relative standard deviation (RSD) ≤ 2.04%, and a recovery range of 98.0-104.3%. The linear range of 5-ASA is 0-120 μM with a LOD of 0.12 μM, RSD ≤ 1.34%, and a recovery range of 98.0-101.3%. When 3-ASA and 5-ASA were mixed in different ratios, the fluorescence showed different colors. The possible mechanism of the interaction between 3-ASA (or 5-ASA) and Ag@BPQD may be ascribed to the generation of excited-state intramolecular proton transfer. To realize convenient detection of 3-ASA and 5-ASA, a Ag@BPQD portable sensing method using cotton swabs were built. The proposed approach provides the detection of 3-ASA and 5-ASA in environmental and biological samples with high efficiency, accuracy and portability.
首次构建了基于新型便携式棉签荧光法的 3-氨基水杨酸（3-ASA）和 5-氨基水杨酸（5-ASA）检测方法。该方法通过在银（Ag）掺杂黑磷量子点（Ag@BPQD）上的荧光增强来实现。Ag@BPQD 是由 AgNO 和块状黑磷在 N，N-二甲基甲酰胺（DMF）溶液中通过机械剥离后的溶剂热分解制备的。Ag@BPQD 具有蓝色荧光，量子产率（QY）为 2.43%。在 Ag@BPQD 的存在下，3-ASA 呈现出明亮的蓝色荧光（λ = 328nm，λ = 448nm）。5-ASA 的荧光也显著增强，呈现出明亮的绿色发射（λ = 328nm，λ = 484nm）。3-ASA 的线性范围为 0-90μM，检测限（LOD）为 0.10μM，相对标准偏差（RSD）≤2.04%，回收率范围为 98.0-104.3%。5-ASA 的线性范围为 0-120μM，LOD 为 0.12μM，RSD≤1.34%，回收率范围为 98.0-101.3%。当 3-ASA 和 5-ASA 以不同比例混合时，荧光呈现出不同的颜色。3-ASA（或 5-ASA）与 Ag@BPQD 相互作用的可能机制可归因于激发态分子内质子转移的产生。为了实现 3-ASA 和 5-ASA 的方便检测，构建了一种使用棉签的 Ag@BPQD 便携式传感方法。该方法为环境和生物样品中 3-ASA 和 5-ASA 的检测提供了高效、准确和便携的方法。
