Pharmaceutics and Pharmacokinetics Division, CSIR-Central Drug Research Institute, Lucknow, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India.
Biomed Chromatogr. 2023 May;37(5):e5600. doi: 10.1002/bmc.5600. Epub 2023 Feb 27.
Fenugreek seeds are used in numerous marketed herbal formulations with therapeutic benefits. Some of its bioactive components such as 4-hydroxyisoleucine, trigonelline, raffinose, and pinitol are reported to possess potential therapeutic activities, such as antibacterial, antidiabetic, stomach stimulant, and anti-invasive, against hyperandrogenism and other allied diseases, including polycystic ovary syndrome. A fully validated, selective, and sensitive bioanalytical method for the simultaneous rapid quantification of the aforementioned bioactive components has been developed using hyphenated liquid chromatography electrospray tandem mass spectrometry. The analytes were separated within 5 min using gradient elution in a C18 column at a flow rate of 0.5 ml/min. Plasma protein precipitation technique was employed to isolate the analytes from the samples. Oral pharmacokinetic profile of the four bioactive components in Sprague-Dawley rats was further evaluated using noncompartmental analysis using Phoenix WinNonlin software.
葫芦巴籽被广泛应用于具有治疗功效的多种市售草药配方中。其一些生物活性成分，如 4-羟基异亮氨酸、三叶草堿、棉子糖和水苏糖，据报道具有潜在的治疗活性，如抗菌、抗糖尿病、胃刺激和抗侵袭，可对抗高雄激素血症和其他相关疾病，包括多囊卵巢综合征。已经开发出一种完全经过验证的、选择性和灵敏的液质联用技术，用于同时快速定量分析上述生物活性成分。在 0.5ml/min 的流速下，使用 C18 柱进行梯度洗脱，可在 5 分钟内分离分析物。采用血浆蛋白沉淀技术从样品中分离分析物。进一步使用 Phoenix WinNonlin 软件的非房室分析方法评估了这四种生物活性成分在 Sprague-Dawley 大鼠中的口服药代动力学特征。
