Shi Yongjie, Xiao Qiwen, Huang Sicong, Pan Qimou, Jia Hongyun, Zhou Qiang, Wei Jie, Kang Jiale, Li Zhe, Hu Yingyu
Department of Clinical Laboratory, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.
J Oncol. 2023 Jan 31;2023:3538928. doi: 10.1155/2023/3538928. eCollection 2023.
The prognosis is poor for hepatocellular carcinoma (HCC), a tumor and cancer associated with inflammation that is common. New data showed that significant levels of KIAA1522 were expressed in HCC tissues and cell lines, suggesting that KIAA1522 may be a highly useful prognostic marker for HCC. However, its biochemical processes and impacts on the immune system go deeper.
To verify the significance of KIAA1522 in HCC and investigate its related carcinogenic mechanisms.
Studies examining the relationship between KIAA1522 expression and clinical-pathologic characteristics in HCC have been checked in the Cancer Genome Atlas (TCGA) database. A receiver operating characteristic (ROC) curve was used to assess the diagnostic efficacy of KIAA1522 in HCC. Western blot analysis was used to find the presence of the KIAA1522 protein in the tumor and paraneoplastic tissues of eight randomly chosen HCC patients. The GSVA program in R language was used to evaluate the relationship between KIAA1522 and immune cell infiltration in HCC. We searched the Search Tool for the Retrieval of Interacting Genes (STRING) database for interacting proteins connected to the expression of KIAA1522. Pathways were involved in the enrichment analysis of KIAA1522 to anticipate potential mechanisms through which KIAA1522 may affect immunological infiltration.
Our study found that KIAA1522 was commonly elevated in HCC tumor tissues and that it also signaled a bad outcome. We found an inverse link between KIAA1522 and cytotoxic cells and an inverse relationship between KIAA1522 and Th2 cell infiltration. In STRING analysis, the top 5 coexpressed proteins of KIAA1522 were BAIAP2, NCK2, TSNAXIP1, POGK, and KLHL31. The effect of KIAA1522 on HCC may entail cell cycle alteration, an immunological response, and suppression of the PPAR signaling pathway.
High expression of KIAA1522 was linked to HCC immune cell infiltration, disease progression, and a poor prognosis.
肝细胞癌（HCC）是一种常见的与炎症相关的肿瘤和癌症，其预后较差。新数据显示，HCC组织和细胞系中KIAA1522表达水平显著，提示KIAA1522可能是HCC一种非常有用的预后标志物。然而，其生化过程及对免疫系统的影响更为深入。
验证KIAA1522在HCC中的意义并探究其相关致癌机制。
在癌症基因组图谱（TCGA）数据库中检索了有关KIAA1522表达与HCC临床病理特征关系的研究。采用受试者工作特征（ROC）曲线评估KIAA1522在HCC中的诊断效能。采用蛋白质印迹分析检测8例随机选取的HCC患者肿瘤及癌旁组织中KIAA1522蛋白的存在情况。使用R语言中的GSVA程序评估KIAA1522与HCC免疫细胞浸润之间的关系。我们在检索相互作用基因的搜索工具（STRING）数据库中查找与KIAA1522表达相关的相互作用蛋白。参与KIAA1522的富集分析以预测KIAA1522可能影响免疫浸润的潜在机制。
我们的研究发现，KIAA1522在HCC肿瘤组织中普遍升高，且预示着不良预后。我们发现KIAA1522与细胞毒性细胞呈负相关，与Th2细胞浸润呈负相关。在STRING分析中，KIAA1522的前5个共表达蛋白为BAIAP2、NCK2、TSNAXIP1、POGK和KLHL31。KIAA1522对HCC的影响可能涉及细胞周期改变、免疫反应以及PPAR信号通路的抑制。
KIAA1522的高表达与HCC免疫细胞浸润、疾病进展及不良预后相关。
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