Weber D M, Fields P A, Romrell L J, Tumwasorn S, Ball B A, Drost M, Fields M J
Department of Animal Science, University of Florida, Gainesville 32611.
Biol Reprod. 1987 Oct;37(3):685-97. doi: 10.1095/biolreprod37.3.685.
This paper describes an in vitro model for the study of two types of steroidogenic luteal cells from cows in different physiological states. Two different populations of enzymatically dispersed bovine luteal cells were separated on the basis of size in a Cel-Sep Sedimentation Chamber. The separated small (12.5-23 micron in diameter) and large (greater than 23 micron in diameter) luteal cells of late-pregnant cows (Days 190-280) contained the distinct morphological characteristics previously defined for these two populations of cells. Cells were evaluated for progesterone (P4) production during a 3-h incubation with and without bovine luteinizing hormone (bLH, 10 ng/ml). Both small and large luteal cells from the late-pregnant cow were found to contain equal levels of P4 at Time 0 and increased but equal levels of P4 after a 3-h incubation. Neither cell type showed an increase in P4 production in response to the addition of bLH (p greater than 0.05). Since these results differed from earlier reports for luteal cells of the nonpregnant cow, small and large luteal cells of the mid-cycle (Day 14) were incubated, and the levels of P4 production were compared with P4 levels from the late pregnant cow. In agreement with previous reports for nonpregnant cows, progesterone content at Time 0 was 7-fold higher in large cells than in small cells (p less than 0.05), and after 3 h of incubation, 13-fold higher (p less than 0.05). Although the small cells responded to the presence of bLH in the incubation medium with a 4-fold increase in P4 production, this increase was not significant (p greater than 0.05). The large cell did not respond to bLH. However, the large cell type continued to contain and produce more P4 than did the small cells treated with bLH. This study indicates that both the small and large luteal cells of late-pregnancy are able to produce P4. However, the large luteal cell of the estrous cycle produces greater quantities of P4 than does the small luteal cell or the large luteal cell of late pregnancy.
本文描述了一种体外模型，用于研究处于不同生理状态的奶牛的两种类固醇生成黄体细胞。在Cel-Sep沉降室中，根据大小对两种不同群体的酶解分散牛黄体细胞进行了分离。妊娠后期（第190 - 280天）分离出的小（直径12.5 - 23微米）大（直径大于23微米）黄体细胞具有先前为这两种细胞群体定义的独特形态特征。在有和没有牛促黄体激素（bLH，10 ng/ml）的情况下孵育3小时期间，对细胞的孕酮（P4）生成进行了评估。发现妊娠后期奶牛的小黄体细胞和大黄体细胞在时间0时P4水平相等，孵育3小时后P4水平升高但仍相等。两种细胞类型在添加bLH后P4生成均未增加（p大于0.05）。由于这些结果与非妊娠奶牛黄体细胞的早期报道不同，对发情周期中期（第14天）的小黄体细胞和大黄体细胞进行了孵育，并将P4生成水平与妊娠后期奶牛的P4水平进行了比较。与非妊娠奶牛的先前报道一致，时间0时大细胞中的孕酮含量比小细胞高7倍（p小于0.05），孵育3小时后高13倍（p小于0.05）。尽管小细胞在孵育培养基中存在bLH时P4生成增加了4倍，但这种增加不显著（p大于0.05）。大细胞对bLH无反应。然而，大细胞类型继续比用bLH处理的小细胞含有和产生更多的P4。这项研究表明，妊娠后期的小黄体细胞和大黄体细胞都能够产生P4。然而，发情周期的大黄体细胞比妊娠后期的小黄体细胞或大黄体细胞产生更多的P4。
