[Effects of miR-144-3p on Proliferation, Cell Cycle and Apoptosis of K562 Cells].

OBJECTIVE

To investigate the effects of miR-144-3p on cell proliferation, cell cycle and apoptosis of blast phase chronic myelogenous leukemia (CML) K562 cells.

METHODS

K562 cells were cultured in vitro and mimics negative control, hsa-miR-144-3p mimics, inhibitor negative control and miR-144-3p inhibitor were respectively transfected into K562 cells with transfection reagents. The cells were divided into five groups including blank control, mimics negative control, miR-144-3p mimics, inhibitor negative control and miR-144-3p inhibitor. After transfection, the cell proliferation activity was detected by CCK-8 assay. The cell cycle distribution and apoptosis were detected by flow cytometry.

RESULTS

Compared with the blank control and mimics negative control groups, the proliferation rate of miR-144-3p mimics group was significantly decreased (<0.05), the proportion of S phase cells was markedly increased (<0.05), while the proportion of G phase cells was obviously decreased (<0.05), and the apoptosis rate was significantly increased (<0.05). Compared with the blank control and inhibitor negative control groups, the proliferation rate of miR-144-3p inhibitor group was obviously increased (<0.05), the proportion of S phase cells was markedly decreased (<0.05), while the proportion of G phase cells was obviously increased (<0.05), and the apoptosis rate was significantly decreased (<0.05).

CONCLUSION

miR-144-3p can inhibit the proliferation and promote apoptosis of K562 cells, affect the cell cycle, and block K562 cells in S phase, which indicates that miR-144-3p is involved in the cell cycle activity of CML during blastic phase.