Aragosa Amina, Saccomanno Benedetta, Specchia Valeria, Frigione Mariaenrica
Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, Italy.
School of Science and Engineering, Al Akhawayn University, Ifrane 53000, Morocco.
Polymers (Basel). 2023 Jan 18;15(3):512. doi: 10.3390/polym15030512.
Polyhydroxybutyrate (PHB) is a biodegradable bio-based polymer synthesized by microorganisms under unfavorable conditions from agro-industrial residues as a source of carbon. These aspects make the bio-based polymer attractive for the mass production of biodegradable plastics, and a definitive replacement for petroleum-based plastics. The aim of this work was to characterize the putative PHB-producing bacterium 1B isolated from the argan soil, to identify the polymer produced, and quantify the PHB production using argan seeds waste. DNA extraction, PCR, and Sanger sequencing were conducted for the molecular identification of strain 1B; the residual biomass and the PHB quantification were measured and compared in the presence of simple sugars and pretreated argan seeds waste. The 1B growth and PHB synthesis were optimized by selecting physical and nutritional parameters: temperature, incubation time, pH, NaCl concentration, and nitrogen sources concentrations. A preliminary characterization of the bio-based polymer extracted was conducted by UV-Visible spectrophotometry and FTIR analysis. The strain 1B was identified as belonging to the genus The PHB final yield was higher in a growth culture enriched with argan waste (3.06%) than with simple sugars. The selected conditions for the bacterial optimal growth incremented the PHB final yield to 6.13%, while the increase in the argan residue concentration from 1 to 3% in a larger culture volume led to the PHB final yield of 8.16%. UV-Visible spectrophotometry of the extracted sample reported a remarkable peak at 248 nm, as well as FTIR spectra analysis, showed peaks at 1728 and 1282 wavenumber/cm. Both preliminary characterizations demonstrated that the extracted sample is the bio-based polymer polyhydroxybutyrate. The results reported in this work reveal how the costless available argan seeds can be used for polyhydroxybutyrate production using a novel species.
聚羟基丁酸酯(PHB)是一种可生物降解的生物基聚合物,由微生物在不利条件下以农业工业残渣作为碳源合成。这些特性使这种生物基聚合物对于大规模生产可生物降解塑料具有吸引力,并且有望最终替代石油基塑料。这项工作的目的是对从摩洛哥坚果树土壤中分离出的假定产PHB细菌1B进行表征,鉴定所产生的聚合物,并使用摩洛哥坚果种子废料对PHB产量进行定量。进行了DNA提取、PCR和桑格测序以对菌株1B进行分子鉴定;在存在单糖和预处理的摩洛哥坚果种子废料的情况下测量并比较了残余生物量和PHB定量。通过选择物理和营养参数(温度、孵育时间、pH、NaCl浓度和氮源浓度)来优化1B的生长和PHB合成。通过紫外可见分光光度法和傅里叶变换红外光谱(FTIR)分析对提取的生物基聚合物进行了初步表征。菌株1B被鉴定为属于 属。在富含摩洛哥坚果废料的生长培养物中,PHB的最终产量(3.06%)高于使用单糖时的产量。所选的细菌最佳生长条件使PHB最终产量提高到6.13%,而在更大的培养体积中将摩洛哥坚果残渣浓度从1%提高到3%,导致PHB最终产量达到8.16%。提取样品的紫外可见分光光度法在248 nm处有一个明显的峰,FTIR光谱分析在波数1728和1282 /cm处有峰。这两种初步表征都表明提取的样品是生物基聚合物聚羟基丁酸酯。这项工作中报告的结果揭示了如何使用一种新的 物种,将免费可得的摩洛哥坚果种子用于聚羟基丁酸酯的生产。