Ali Ramadan, El-Wekil Mohamed M
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Tabuk, Tabuk 71491, Saudi Arabia; Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Al Azhar University, Assiut Branch 71526, Egypt.
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt.
Food Chem. 2023 Jul 1;413:135666. doi: 10.1016/j.foodchem.2023.135666. Epub 2023 Feb 10.
A synergistic hybrid was fabricated for the electrochemical aptasensing of acrylamide (AAM) via molecularly imprinted technology. The aptasensor depends on the modification of glassy carbon electrode with AuNPs and reduced graphene oxide (rGO)/multiwalled carbon nanotubes (MWCNTs) {Au@rGO-MWCNTs/GCE}. The aptamer (Apt-SH) and AAM (template) were incubated with the electrode. After that, the monomer was electro-polymerized to fabricate molecular imprinted polymeric film (MIP) over the surface of Apt-SH/Au@rGO/MWCNTs/GCE. The modified electrodes were characterized using different morphological and electrochemical techniques. Under optimum conditions, the aptasensor exhibited a linear relationship between AAM concentration and anodic peak current difference (ΔIpa) in the range of 1-600 nM with a limit of quantitation (LOQ, S/N = 10) and a limit of detection (LOD, S/N = 3) of 0.346 and 0.104 nM, respectively. The aptasensor was successfully applied for the determination of AAM in potato fries samples with recoveries % in the range of 98.7-103.4 % and RSDs did not exceed 3.2 %. The advantages of MIP/Apt-SH/Au@rGO/MWCNTs/GCE are low detection limit, high selectivity, and satisfactory stability towards AAM detection.
通过分子印迹技术制备了一种用于丙烯酰胺（AAM）电化学适配体传感的协同杂化物。该适配体传感器依赖于用金纳米颗粒（AuNPs）和还原氧化石墨烯（rGO）/多壁碳纳米管（MWCNTs）{Au@rGO-MWCNTs/GCE}修饰玻碳电极。将适配体（Apt-SH）和AAM（模板）与电极孵育。之后，将单体进行电聚合，在Apt-SH/Au@rGO/MWCNTs/GCE表面制备分子印迹聚合物膜（MIP）。使用不同的形态学和电化学技术对修饰电极进行表征。在最佳条件下，该适配体传感器在1-600 nM范围内AAM浓度与阳极峰电流差（ΔIpa）之间呈现线性关系，定量限（LOQ，S/N = 10）和检测限（LOD，S/N = 3）分别为0.346和0.104 nM。该适配体传感器成功应用于薯条样品中AAM的测定，回收率在98.7-103.4%范围内，相对标准偏差（RSD）不超过3.2%。MIP/Apt-SH/Au@rGO/MWCNTs/GCE的优点是检测限低、选择性高以及对AAM检测具有令人满意的稳定性。
