Polystyrene nanoplastics' accumulation in roots induces adverse physiological and molecular effects in water spinach Ipomoea aquatica Forsk.

The ubiquity of plastic pollution has emerged as a perplexing issue for aquatic and terrestrial plants. To assess the toxic effects of polystyrene NPs (PS-NPs, 80 nm), we conducted a hydroponic experiment in which water spinach (Ipomoea aquatica Forsk) was subjected to low (0.5 mg/L), medium (5 mg/L), and high (10 mg/L) concentrations of fluorescent PS-NPs for 10 days to examine their accumulation and transportation in water spinach and associated impacts on growth, photosynthesis, antioxidant defense systems. Laser confocal scanning microscopy (LCSM) observations at 10 mg/L PS-NPs exposure indicated that PS-NPs only adhered to the root surface of water spinach and were not transported upward, indicating that short-term exposure to high concentrations of PS-NPs (10 mg/L) did not cause the internalization of PS-NPs in the water spinach. However, this high concentration of PS-NPs (10 mg/L) discernibly inhibited the growth parameters (fresh weight, root length and shoot length), albeit failed to induce any significant impact on chlorophyll a and chlorophyll b concentrations. Meanwhile, high concentration of PS-NPs (10 mg/L) significantly decreased the SOD and CAT activities in leaves (p < 0.05). At the molecular level, low and medium concentrations of PS-NPs (0.5, 5 mg/L) significantly promoted the expression of photosynthesis (PsbA and rbcL) and antioxidant-related (SIP) genes in leaves (p < 0.05), and high concentration of PS-NPs (10 mg/L) significantly increased the transcription levels of antioxidant-related (APx) genes (p < 0.01). Our results imply that PS-NPs accumulate in the roots of water spinach, compromising the upward transport of water and nutrients and undermining the antioxidant defense system of the leaves at the physiological and molecular levels. These results provide a fresh perspective to examine the implications of PS-NPs on edible aquatic plants, and future efforts should be focused intensively on the impacts of PS-NPs on agricultural sustainability and food security.