利用灵敏的气相色谱 - 质谱法分析马血、血浆和尿液样本中的地托咪定。

Analysis of detomidine in horse blood, plasma and urine samples utilizing a sensitive gas chromatography-mass spectrometry method.

作者信息

Singh A K, Mishra U, Ashraf M, Abdennebi E H, Granley K, Dombrovskis D, Hewetson D, Stowe C M

机构信息

Department of Veterinary Diagnostic Investigation, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.

出版信息

J Chromatogr. 1987 Aug 28;404(1):223-32. doi: 10.1016/s0021-9673(01)86852-7.

DOI:10.1016/s0021-9673(01)86852-7

PMID:3680434

Abstract

Chemical ionization- and electron impact ionization-selective ion monitoring provided a simple and sensitive method for measuring detomidine (Domosedan), a potent sedative-analgesic drug for horses and cattle. Chemical ionization was at least 10 times more sensitive than electron impact ionization. By using propranolol as an internal standard, we found that the recovery of detomidine from the extraction procedure used in this study was greater than 75% for plasma, whole blood, or urine samples. Approximately 68% of detomidine was bound to plasma protein and 53% was bound to red blood cells.

摘要

化学电离和电子轰击电离选择性离子监测为测量地托咪定（多咪静）提供了一种简单且灵敏的方法，地托咪定是一种用于马和牛的强效镇静镇痛药。化学电离的灵敏度至少比电子轰击电离高10倍。通过使用普萘洛尔作为内标，我们发现，在本研究中所用的提取程序中，血浆、全血或尿液样本中地托咪定的回收率大于75%。约68%的地托咪定与血浆蛋白结合，53%与红细胞结合。