Ünaldı Ayça Şeyma, Çubuk Soner, Çiğil Aslı Beyler, Kahraman M Vezir
Chemistry Department, Faculty of Science, Marmara University, 34722, Istanbul, Türkiye.
Dep. of Chemistry and Chemical Process Technology School, Amasya University Technical Sci. Vocational, Amasya, Türkiye.
J Fluoresc. 2023 Jul;33(4):1651-1659. doi: 10.1007/s10895-023-03181-8. Epub 2023 Feb 20.
In this study, with the help of reactive monomers, crosslinkers, and photoinitiator that detect HS in various matrices, an HS sensitive fluorescence sensor polymerizes under ultraviolet (UV) light was developed. To this goal, a polymeric membrane was prepared, and the characterization of the membrane was carried out with Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) methods. Afterward, appropriate conditions were identified, the excitation wavelength was determined as 370 nm, and the emission wavelength was determined as 425 nm. It was established that the fluorescence intensity of the prepared polymeric membrane decreased in the presence of HS. A detailed analysis was executed to determine the sensor's most suitable pH value and time. It was found that the optimum pH was 8.0, and the optimal duration was 15 s. It has been calculated that the linear range of the developed method is 2.19 × 10- 6.25 × 10 M, and the detection limit (LOD) is 7.37 × 10 M. The effect of some possible interfering ions was investigated, and it determined that the sensor had excellent selectivity. In addition, the sensor used to determine HS can be used at least 100 times. The recovery percentages were 102.1%-103.2%, and 104.6%, using tap water samples. In terms of providing reliable, fast results, high sensitivity, reusable, low cost, and ease of use, the developed fluorimetric sensor, compared to standard methods, has become more advantageous.
在本研究中，借助于可检测各种基质中HS的反应性单体、交联剂和光引发剂，开发了一种在紫外光下聚合的HS敏感荧光传感器。为此，制备了聚合物膜，并采用傅里叶变换红外光谱（FTIR）和扫描电子显微镜（SEM）方法对膜进行了表征。随后，确定了合适的条件，激发波长确定为370nm，发射波长确定为425nm。结果表明，制备的聚合物膜在HS存在下荧光强度降低。进行了详细分析以确定传感器最合适的pH值和时间。发现最佳pH值为8.0，最佳持续时间为15秒。经计算，所开发方法的线性范围为2.19×10 - 6.25×10 M，检测限（LOD）为7.37×10 M。研究了一些可能干扰离子的影响，结果表明该传感器具有优异的选择性。此外，用于测定HS的传感器至少可使用100次。使用自来水样品时，回收率分别为102.1%-103.2%和104.6%。与标准方法相比，所开发的荧光传感器在提供可靠、快速的结果、高灵敏度、可重复使用、低成本和易于使用方面更具优势。
