Tanaka T, Araki E, Nitta K, Tateno M
Pharmacology Division, National Cancer Center Research Institute and Hospital, Tokyo, Japan.
J Biol Response Mod. 1987 Oct;6(5):484-8.
Recently many findings about the physiological and biochemical functions of recombinant human tumor necrosis factor (rHu-TNF) have been reported. In the present study, the effect of rHu-TNF on serum iron in C3H/HeN and C57BL/6 mice was determined. Blood samples were obtained before intravenous injection of rHu-TNF, and also at various times after the injection. Results from five experiments showed that the serum iron was depressed between 1/3 to 1/5 of that of untreated mice 4 to 24 h after injection of 5 or 10 micrograms of rHu-TNF. The low level persisted for 33 h, rebounded at 48 to 72 h, and returned to normal by 96 h after the injection. Serum iron was determined by the colorimetric method using the sensitive reagent, ferrozine (3-(2-pyridyl)-5,6-bis(4-sulfophenyl)-1,2,4-triazine. We believe that this is the first report of depression of serum iron in mice by intravenous injection of rHu-TNF. The physiological role of repressed serum iron in the in vivo response to TNF remains to be established.
最近，关于重组人肿瘤坏死因子（rHu-TNF）生理生化功能的许多研究结果已被报道。在本研究中，测定了rHu-TNF对C3H/HeN和C57BL/6小鼠血清铁的影响。在静脉注射rHu-TNF之前以及注射后的不同时间采集血样。五个实验的结果表明，注射5或10微克rHu-TNF后4至24小时，血清铁水平降至未处理小鼠的1/3至1/5。低水平持续33小时，在48至72小时反弹，并在注射后96小时恢复正常。血清铁采用比色法，使用灵敏试剂亚铁嗪（3-（2-吡啶基）-5,6-双（4-磺基苯基）-1,2,4-三嗪）进行测定。我们认为，这是首次关于静脉注射rHu-TNF导致小鼠血清铁降低的报道。血清铁降低在体内对TNF反应中的生理作用仍有待确定。
