剑麻汁水提取物显著的抗血脂异常、降血糖、抗高血糖和抗肥胖活性部分是通过调节钙信号通路介导的。

The significant antidyslipidemic, hypoglycemic, antihyperglycemic, and antiobesity activities of the aqueous extracts of Agave Sisalana juice are partly mediated via modulation of calcium signaling pathways.

作者信息

Chege Boniface Mwangi, Nyaga Nelly Murugi, Kaur Prabjot Sehmi, Misigo Wycliffe Odhiambo, Khan Nelson, Wanyonyi Wycliffe Chisutia, Mwangi Peter Waweru

机构信息

Dedan Kimathi University of Technology, School of Health Sciences, Kenya.

University of Nairobi, Department of Medical Physiology, Kenya.

出版信息

Heliyon. 2022 Dec 24;9(2):e12400. doi: 10.1016/j.heliyon.2022.e12400. eCollection 2023 Feb.

DOI:10.1016/j.heliyon.2022.e12400

PMID:36816233

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9932365/

Abstract

Plant species in the genus Agave, including , have found extensive application in African and Asian traditional medicine. Inspired by the use of the edible sweet sap known as Aguamiel (obtained from specific mature agave species such as ) in Mexico by diabetic patients to improve their diabetic condition, this study investigated the effects of extracts prepared by lyophilization, fermentation, and saponin extraction from sisal juice in a rodent model of metabolic syndrome. The metabolic syndrome was induced by administering a high fat and high fructose diet to freshly weaned Sprague-Dawley rats for eight weeks. The extracts possessed significant hypoglycemic effects [3.883 ± 0.371 mmol/L (normal group) vs. 8.183 ± 0.5845 mmol/L (negative control) vs. 3.767 ± 0.2716 mmol/L (positive control) vs. 4.167 ± 0.4602 mmol/L (FSP) vs. 4.533 ± 0.3169 mmol/L (FerSP) vs. 3.5 ± 0.2309 mmol/L (FS LD) vs. 3.867 ± 0.3353 mmol/L (FS HD) vs. 4.617 ± 0.2725 mmol/L (FerS LD) vs. 4.383 ± 0.3114 mmol/L (FerS HD): p < 0.0001]. The extracts also possessed significant antihyperlipidemic effects with significant differences in total serum cholesterol between the groups [1.398 ± 0.1232 mmol/L (normal group) vs. 4.225 ± 0.4135 mmol/L (negative control) vs. 1.582 ± 0.154 mmol/L (positive control) vs. 1.245 ± 0.0911 mmol/L (FSP) vs. 1.393 ± 0.1423 mmol/L (FerSP) vs. 1.387 ± 0.0924 mmol/L (FS LD) vs. 1.761 ± 0.1495 mmol/L (FS HD) vs. 1.698 ± 0.1294 mmol/L (FerS LD) vs. 1.6975 ± 0.0982 mmol/L (FerS HD): p < 0.0001]. Further, significant antiobesity effects of the extracts were observed with significant differences in weight among the groups [196.3 ± 6.49 g (normal group) vs. 298.9 ± 6.67 g (negative control) vs. 215.3 ± 6.06 g (positive control) vs. 195.4 ± 3.92 g (FSP) vs. 213.1 ± 5.21 g (FerSP) vs. 190.8 ± 6.49 g (FS LD) vs. 198.9 ± 4.31 g (FS HD) vs. 204.7 ± 4.78 g (FerS LD) vs. 208.7 ± 6.21 g (FerS HD): p < 0.0001]. Network pharmacology studies indicated that the chemical components found in sisal juice primarily exert their effects by modulating the voltage-gated calcium channels CACNA1S, CACNA1D, and CACNA1C, in the beta cells of the islets of Langerhans.

摘要

龙舌兰属的植物物种，包括，在非洲和亚洲传统医学中有着广泛应用。受墨西哥糖尿病患者使用名为阿瓜蜜（从特定成熟龙舌兰物种如中获取）的可食用甜汁液来改善糖尿病病情的启发，本研究在代谢综合征啮齿动物模型中研究了通过冻干、发酵和从剑麻汁中提取皂苷制备的提取物的效果。通过给刚断奶的斯普拉格-道利大鼠喂食高脂肪和高果糖饮食八周来诱导代谢综合征。提取物具有显著的降血糖作用[3.883±0.371毫摩尔/升（正常组）对8.183±0.5845毫摩尔/升（阴性对照组）对3.767±0.2716毫摩尔/升（阳性对照组）对4.167±0.4602毫摩尔/升（FSP）对4.533±0.3169毫摩尔/升（FerSP）对3.5±0.2309毫摩尔/升（FS LD）对3.867±0.3353毫摩尔/升（FS HD）对4.617±0.2725毫摩尔/升（FerS LD）对4.383±0.3114毫摩尔/升（FerS HD）：p<0.0001]。提取物还具有显著的抗高血脂作用，各组之间总血清胆固醇存在显著差异[1.398±0.1232毫摩尔/升（正常组）对4.225±0.4135毫摩尔/升（阴性对照组）对1.582±0.154毫摩尔/升（阳性对照组）对1.245±0.0911毫摩尔/升（FSP）对1.393±0.1423毫摩尔/升（FerSP）对1.387±0.0924毫摩尔/升（FS LD）对1.761±0.1495毫摩尔/升（FS HD）对1.698±0.1294毫摩尔/升（FerS LD）对1.6975±0.0982毫摩尔/升（FerS HD）：p<0.0001]。此外，观察到提取物具有显著的抗肥胖作用，各组之间体重存在显著差异[196.3±6.49克（正常组）对298.9±6.67克（阴性对照组）对215.3±6.06克（阳性对照组）对195.4±3.92克（FSP）对213.1±5.21克（FerSP）对190.8±6.49克（FS LD）对198.9±4.31克（FS HD）对204.7±4.78克（FerS LD）对208.7±6.21克（FerS HD）：p<0.0001]。网络药理学研究表明，剑麻汁中发现的化学成分主要通过调节胰岛β细胞中的电压门控钙通道CACNA1S、CACNA1D和CACNA1C发挥作用。