多组学联合分析揭示了[具体植物名称]花色和香气形成的潜在机制。需注意，你提供的原文中“in.”后面缺少具体内容，我根据语境补充了“[具体植物名称]”，以便译文更完整。

Combined analysis of multi-omics reveals the potential mechanism of flower color and aroma formation in .

作者信息

Wang Yonggui, Xia Jing, Wang Zile, Ying Zhiping, Xiong Zhi, Wang Changming, Shi Rui

机构信息

Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, International Ecological Forestry Research Center of Kunming, Southwest Forestry University, Kunming, China.

Yunnan Agricultural University College of Plant Protection, Kunming, China.

出版信息

Front Plant Sci. 2023 Feb 1;13:1095644. doi: 10.3389/fpls.2022.1095644. eCollection 2022.

DOI:10.3389/fpls.2022.1095644

PMID:36816481

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9931397/

Abstract

INTRODUCTION

Maiden & Betche is a domesticated high-value nut crop. The development of nut flower affects the fruit setting rate, yield and quality of nuts. Therefore, in this experiment, two varieties with different flower color, flowering time, flowering quantity and nut yield (single fruit weight) were selected as the research objects.

METHODS

Transcriptome (RNA-Seq) and metabolome (LC-MS/MS, GC-MS) analyses were performed to study the regulatory mechanisms of nut flower development, color and aroma.

RESULTS

The results indicated that plant hormone signal transduction, starch sucrose metabolism, phenylpropanoid metabolism, flavonoid biosynthesis, and anthocyanin biosynthesis pathways were related to nut flower development and flower color formation. In the early stage of flowering, most of the differentially expressed genes (DEGs) are involved in the IAA signal transduction pathway, while in the later stage, the brassinolide signal pathway is mainly involved. In starch and sugar metabolism, DEGs are mainly involved in regulating and hydrolyzing stored starch into small molecular sugars in flower tissues. In the phenylpropanoid biosynthesis pathway, DEGs are mainly related to the color and aroma (volatile organic compounds, VOCs) formation of nut flowers. Four color formation metabolites (anthocyanins) in nut flowers were found by LC-MS/MS detection. In addition, the VOCs showed no significant difference between red nut flowers (R) and white nut flowers (W), which was mainly reflected in the aroma formation stage (flowering time). And 12 common differentially accumulation metabolites (DAMs) were detected by GC-MS and LC-MS/MS. At the same time, the DEGs, , and genes, were also identified to regulate key metabolite synthesis during nut flower development. These genes were further verified by qRT-PCR.

CONCLUSION

Our results provide insights to clarify the molecular mechanism of color and aroma formation during flower development that pave the way for nut quality and yield breeding.

摘要

引言

澳洲坚果是一种驯化的高价值坚果作物。坚果花的发育影响着坐果率、产量和坚果品质。因此，在本实验中，选择了两个在花色、开花时间、开花数量和坚果产量（单果重）方面存在差异的品种作为研究对象。

方法

进行转录组（RNA测序）和代谢组（液相色谱-质谱联用、气相色谱-质谱联用）分析，以研究坚果花发育、颜色和香气的调控机制。

结果

结果表明，植物激素信号转导、淀粉蔗糖代谢、苯丙烷代谢、类黄酮生物合成和花青素生物合成途径与坚果花发育和花色形成有关。在开花早期，大多数差异表达基因参与生长素信号转导途径，而在后期，主要涉及油菜素内酯信号途径。在淀粉和糖代谢中，差异表达基因主要参与将花组织中储存的淀粉调节并水解为小分子糖。在苯丙烷生物合成途径中，差异表达基因主要与坚果花的颜色和香气（挥发性有机化合物）形成有关。通过液相色谱-质谱联用检测发现坚果花中有四种颜色形成代谢物（花青素）。此外，红色坚果花（R）和白色坚果花（W）之间的挥发性有机化合物在香气形成阶段（开花时间）没有显著差异。通过气相色谱-质谱联用和液相色谱-质谱联用检测到12种常见的差异积累代谢物。同时，还鉴定出差异表达基因、和基因在坚果花发育过程中调节关键代谢物的合成。这些基因通过实时定量聚合酶链反应进一步验证。