Sun Meili, Wang Hancheng, Shi Caihua, Li Jianjun, Cai Liuti, Xiang Ligang, Liu Tingting, Goodwin Paul H, Chen Xingjiang, Wang Ling
College of Agriculture, Yangtze University, Jingzhou, Hubei, China.
Guizhou Provincial Academician Workstation of Microbiology and Health, Guizhou Academy of Tobacco Science, Guiyang, China.
Front Plant Sci. 2023 Feb 1;13:1101039. doi: 10.3389/fpls.2022.1101039. eCollection 2022.
Azoxystrobin, a quinone outside inhibitor fungicide, reduced tobacco target spot caused by by 62%, but also affected the composition and diversity of other microbes on the surface and interior of treated tobacco leaves. High-throughput sequencing showed that the dominant bacteria prior to azoxystrobin treatment were on healthy leaves and on diseased leaves, and the dominant fungi were (teleomorph of ) and on healthy leaves and on diseased leaves. Both bacterial and fungal diversity significantly increased 1 to 18 days post treatment (dpt) with azoxystrobin for healthy and diseased leaves. For bacteria on healthy leaves, the relative abundance of , and declined, while and increased. On diseased leaves, the relative abundance of and declined, while and increased. For fungi on healthy leaves, the relative abundance of declined, while , , , and increased. On diseased leaves, the relative abundance of declined, while , , , , , and , increased. Compared to healthy leaves, azoxystrobin treatment of diseased leaves resulted in greater reductions in , and , a greater increase in , and similar changes in , and . Azoxystrobin had a semi-selective effect altering the microbial diversity of the tobacco leaf microbiome, which could be due to factors, such as differences among bacterial and fungal species in sensitivity to quinone outside inhibitors, ability to use nutrients and niches as certain microbes are affected, and metabolic responses to azoxystrobin.
嘧菌酯，一种醌外抑制剂类杀菌剂，使烟草靶斑病的发病率降低了62%，但也影响了处理过的烟草叶片表面和内部其他微生物的组成和多样性。高通量测序显示，在嘧菌酯处理之前，健康叶片上的优势细菌是 ，患病叶片上的优势细菌是 ，健康叶片上的优势真菌是 （ 的有性型）和 ，患病叶片上的优势真菌是 。对于健康和患病叶片，在使用嘧菌酯处理后的1至18天（dpt），细菌和真菌的多样性均显著增加。对于健康叶片上的细菌， 、 和 的相对丰度下降，而 和 的相对丰度增加。在患病叶片上， 和 的相对丰度下降，而 和 的相对丰度增加。对于健康叶片上的真菌， 的相对丰度下降，而 、 、 、 和 的相对丰度增加。在患病叶片上， 的相对丰度下降，而 、 、 、 、 、 和 的相对丰度增加。与健康叶片相比，用嘧菌酯处理患病叶片导致 、 和 的减少幅度更大， 的增加幅度更大，而 、 和 的变化相似。嘧菌酯对烟草叶微生物群的微生物多样性具有半选择性影响，这可能是由于细菌和真菌物种对醌外抑制剂的敏感性差异、某些微生物受到影响时利用营养物质和生态位的能力以及对嘧菌酯的代谢反应等因素造成的。
