Department of Food Commodity ScienceDepartment of Food Commodity Science, Faculty of Health Sciences with the Institute of Maritime and Tropical Medicine, Medical University of Gdansk, ul. Debinki 7, 80-211, Gdansk, Poland.
Department of General, Endocrine, and Transplant Surgery, Faculty of Medicine, Medical University of Gdansk, ul. Smoluchowskiego 17, 80-214, Gdansk, Poland.
Obes Surg. 2023 Apr;33(4):1228-1236. doi: 10.1007/s11695-023-06518-1. Epub 2023 Feb 25.
Obesity is associated with disturbed gut microbiota homeostasis that translates into altered intestinal and blood metabolite profiles. The long-chain fatty acid (LCFA) may be absorbed in the intestine, but until now, their composition in intestinal contents of patients with obesity has not been studied. The aim of the present study was to verify whether obesity is related to any changes in fecal LCFA content and whether intestinal LCFA content may be associated with the health status of patients with obesity.
The fatty acid composition has been studied in stool samples obtained from 26 patients with morbid obesity and 25 lean subjects by gas chromatography-mass spectrometry. The dietary habits were assessed using the Food Frequency Questionnaire (FFQ-6).
Our results show for the first time that lean subjects and patients with obesity differ in their stool LCFA profiles. The levels of most n-3 polyunsaturated fatty acids (PUFAs) and n-6 PUFAs were significantly higher in fecal samples from people with obesity than in those from lean controls.
Based on the current knowledge, we have defined three hypotheses that may explain proving the cause-and-effect relationships observed differences in fecal LCFA profiles between patients with obesity and lean subjects. They may be related to alterations in fat digestion and/or LCFA absorption and diet. However, proving the cause-and-effect relationships requires further research.
肥胖与肠道微生物群稳态失调有关，这会导致肠道和血液代谢物谱发生变化。长链脂肪酸（LCFA）可能在肠道中被吸收，但到目前为止，肥胖患者肠道内容物中的 LCFA 组成尚未得到研究。本研究旨在验证肥胖是否与粪便 LCFA 含量的任何变化有关，以及肠道 LCFA 含量是否与肥胖患者的健康状况有关。
通过气相色谱-质谱法研究了 26 名病态肥胖患者和 25 名正常体重受试者的粪便样本中的脂肪酸组成。使用食物频率问卷（FFQ-6）评估饮食习惯。
我们的研究结果首次表明，瘦人和肥胖患者的粪便 LCFA 谱存在差异。与瘦对照组相比，肥胖患者粪便中大多数 n-3 多不饱和脂肪酸（PUFA）和 n-6 PUFA 的水平明显更高。
根据目前的知识，我们已经定义了三个假设，这些假设可以解释为什么肥胖患者和瘦对照组的粪便 LCFA 谱存在差异。这些差异可能与脂肪消化和/或 LCFA 吸收和饮食的改变有关。然而，要证明这些因果关系，还需要进一步的研究。
