Grassi Andrea, Gambini Matteo, Pantoli Marianna, Toscano Simona, Albertetti Anna, Del Frassino Deborah Maria, Ugochukwu Iniobong Chukwuebuka Ikenna, Romeo Orazio, Otranto Domenico, Cafarchia Claudia
I-Vet Diagnostica Veterinaria, 25020 Brescia, Italy.
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, 27100 Pavia, Italy.
J Fungi (Basel). 2023 Feb 1;9(2):191. doi: 10.3390/jof9020191.
infections have a poor prognosis in animals, most likely due to a lack of knowledge about diagnosis and treatment. In this study, we described a case of a lethal infection in a captive bullfrog () in Europe. One adult male bullfrog was referred with clinical signs of lethargy and a cutaneous nodule. Fungal infection was suspected on cytology and confirmed by histology and cultural isolation. The mold was identified by molecular methods using partial sequencing of the TEF1α gene and the ITS region of rDNA. Climbazole antifungal treatment was started but the frog died after 30 days, and necropsy was done. Pigmented hyphae and structures consistent with muriform bodies were found on a background of diffuse granulomatous inflammation at cytological and histopathological examinations. Fungal culture revealed the presence of pigmented fungi identified as only by partial sequencing of the TEF1α gene. A focally extensive granuloma with intralesional hyphae and muriform bodies effacing the architecture of head, liver, kidneys, lungs, and large intestine were retrieved after necropsy. This study is the first Italian report of the occurrence of lethal infection in a frog and highlights the role of this sp. in chromoblastomycosis.
感染在动物中预后较差，很可能是由于对诊断和治疗缺乏了解。在本研究中，我们描述了欧洲一只圈养牛蛙发生致命感染的病例。一只成年雄性牛蛙因嗜睡和皮肤结节的临床症状被转诊。细胞学检查怀疑为真菌感染，并通过组织学和培养分离得到证实。使用TEF1α基因的部分测序和rDNA的ITS区域，通过分子方法鉴定了该霉菌。开始使用克霉唑进行抗真菌治疗，但青蛙在30天后死亡，并进行了尸检。在细胞学和组织病理学检查中，在弥漫性肉芽肿性炎症背景下发现了色素菌丝和与砖格状小体一致的结构。真菌培养显示存在仅通过TEF1α基因部分测序鉴定为的色素真菌。尸检后发现一个局灶性广泛的肉芽肿，病灶内有菌丝和砖格状小体，破坏了头部、肝脏、肾脏、肺和大肠的结构。本研究是意大利关于青蛙发生致命感染的首次报告，并强调了该种在着色芽生菌病中的作用。
