Zhang Ying, Chen Ying, Xu Ke, Xia Siyu, Aihaiti Ailifeire, Zhu Mingxia, Wang Chonggang
State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, People's Republic of China.
Environ Sci Pollut Res Int. 2023 Apr;30(18):52369-52379. doi: 10.1007/s11356-023-26165-4. Epub 2023 Feb 25.
To explore the impact of embryonic exposure to phenanthrene (Phe), a typical tricyclic polycyclic aromatic hydrocarbon, on cardiac development in next generation, fertilized zebrafish embryos were exposed to 0.05, 0.5, 5 and 50 nM Phe for 96 h, and then transferred to clear water and raised to adulthood. The cardiac development in F1 larvae generated by adult females or males mated with unexposed zebrafish was assessed. Malformation and dysfunction of the heart, such as increased heart rate, arrhythmia, enlarged heart and abnormal contraction, were shown in both paternal and maternal F1 larvae. A greater impact on the distance between the sinus venosus and bulbus arteriosus was exhibited in maternal F1 larvae, while paternal F1 larvae displayed a more severe impact on heart rate and arrhythmia. The transcription of genes related to cardiac development was disturbed in F1 larvae. DNA methylation levels in the promoter of some genes were associated with their transcription. The expression of acetylated histone H3K9Ac and H3K14Ac in maternal F1 larvae was no significantly changed, but was significantly downregulated in paternal F1 larvae, which might be associated with the downregulated transcription of tbx5. These results indicate that exposure to Phe during embryogenesis adversely affects cardiac development in F1 generation, and the effects and toxic mechanisms showed sex-linked hereditary differences, highlighting the risk of Phe exposure in early life to heart health in next generation.
为探究胚胎期暴露于典型三环多环芳烃菲（Phe）对下一代心脏发育的影响，将受精斑马鱼胚胎暴露于0.05、0.5、5和50 nM的Phe中96小时，然后转移至清水中饲养至成年。评估与未暴露斑马鱼交配的成年雌性或雄性产生的F1幼体的心脏发育情况。父本和母本F1幼体均出现心脏畸形和功能障碍，如心率加快、心律失常、心脏增大和收缩异常。母本F1幼体中窦房结与动脉球之间的距离受到的影响更大，而父本F1幼体中心率和心律失常受到的影响更严重。F1幼体中与心脏发育相关基因的转录受到干扰。一些基因启动子中的DNA甲基化水平与其转录相关。母本F1幼体中乙酰化组蛋白H3K9Ac和H3K14Ac的表达无显著变化，但在父本F1幼体中显著下调，这可能与tbx5转录下调有关。这些结果表明，胚胎期暴露于Phe会对F1代心脏发育产生不利影响，且其影响和毒性机制存在性别连锁遗传差异，凸显了生命早期暴露于Phe对下一代心脏健康的风险。
