Membrane bioreactors (MBRs) suffer from high operational and cleaning costs due to biofouling. The biofouling begins when the adhesins (an anchor-type epitope made up of polar and charged amino acids) on microbial appendages bind to the surface. Two different compounds-dodecyl-β-D-maltoside (DDM) and methyl α-d-mannopyranoside (MeαMan)-were investigated as possible biofilm mitigation tools due to their documented anti-adhesin properties in the biomedical field. DDM prevented up to 56.3, 87.0, and 67.6% of the formation of Pseudomonas putida, Escherichia coli and wastewater culture biofilms, respectively, in microplate experiments. MeαMan increased biofilm in the microplates. In a biofilm reactor setting, DDM was then applied on typical membrane materials, polyvinylidene fluoride, polyamide, polyether-sulfone, and polyacrylonitrile and prevented 79.4, 62.5, 81.3, and 68.2% of the detectable wastewater culture biofilm formation, respectively. The mechanism of anti-adhesion was the binding of the polar head of the DDM to the polar amino acids of the microbial appendages in conjunction with the orientation of the DDM as it binds different membrane materials. If the anti-adhesins are effective at increasing the distance of the bacteria from the membrane materials, they will serve as a new method for delaying biofouling.