Sahyon Heba A, El-Shafai Nagi M, El-Mehasseb Ibrahim, Althobaiti Fayez, Aldhahrani Adil, Elnajjar Noha
Chemistry Department, Faculty of Science, Kafrelsheikh University, 33516 Kafrelsheikh, Egypt.
Nanotechnology Center, Chemistry Department, Faculty of Science, Kafrelsheikh University, 33516, Egypt.
Int J Biol Macromol. 2023 Apr 30;235:123804. doi: 10.1016/j.ijbiomac.2023.123804. Epub 2023 Feb 24.
The liver is the most important organ in the body. Hepatocyte oxidative damage occurs to excess ROS. Liver fibrosis is a mechanism that the immune system uses to treat extreme inflammation by repairing damaged tissue with the creation of a scar. The outcome of fibrosis may be reversed by consuming natural plant extracts with high ROS-scavenging ability. The date palm fruits contain caffeic acid, gallic acid, syringic acid, and ferulic acid, which have anti-inflammatory, antioxidant, and hepatoprotective properties. This study aimed to prepare a date fruit extract, load it onto chitosan nanoparticles, and compare its anti-fibrotic activity with the unloaded crude extract in the CCl-mouse model. Our findings show that nanocomposite (Cs@FA/DEx) has anti-fibrotic properties and can improve liver function enzymes and endogenous antioxidant enzymes by inhibiting cell apoptosis caused by CCl-induction in mice. Furthermore, significantly reduced CD95 and ICAM1 levels and down-regulation of TGFβ-1 and collagen-α-1 expression demonstrated the anti-fibrotic effects of the Cs@FA/DEx. Therefore, the Cs@FA/DEx might be an innovative supplement for inhibiting liver fibrosis and hepatocyte inflammation induced by chemical toxins. Besides, this nano-supplement could be a promising anti-hepatocellular carcinoma agent as it has potent in vitro anticancer activity against the HePG2 cell line.
肝脏是人体最重要的器官。过量的活性氧（ROS）会导致肝细胞氧化损伤。肝纤维化是免疫系统通过形成瘢痕修复受损组织来应对极度炎症的一种机制。食用具有高ROS清除能力的天然植物提取物可能会逆转纤维化的结果。枣椰果含有咖啡酸、没食子酸、丁香酸和阿魏酸，这些物质具有抗炎、抗氧化和肝脏保护特性。本研究旨在制备枣果提取物，将其负载到壳聚糖纳米颗粒上，并在CCl4诱导的小鼠模型中比较其与未负载的粗提物的抗纤维化活性。我们的研究结果表明，纳米复合材料（Cs@FA/DEx）具有抗纤维化特性，并且可以通过抑制CCl4诱导的小鼠细胞凋亡来改善肝功能酶和内源性抗氧化酶。此外，CD95和ICAM1水平显著降低以及TGFβ-1和胶原蛋白-α-1表达下调证明了Cs@FA/DEx的抗纤维化作用。因此，Cs@FA/DEx可能是一种抑制化学毒素诱导的肝纤维化和肝细胞炎症的创新补充剂。此外，这种纳米补充剂可能是一种有前景的抗肝细胞癌药物，因为它对HePG2细胞系具有强大的体外抗癌活性。
