Salvia Roser, Rico Laura G, Bradford Jolene A, Ward Michael D, Olszowy Michael W, Martínez Cristina, Madrid-Aris Álvaro Domingo, Grífols Joan R, Ancochea Águeda, Gomez-Muñoz Laia, Vives-Pi Marta, Martínez-Cáceres Eva, Fernández Marco A, Sorigue Marc, Petriz Jordi
Germans Trias i Pujol Research Institute (IGTP), ICO-Hospital Germans Trias i Pujol, Universitat Autònoma de Barcelona (UAB), Badalona, Barcelona, Spain.
Department of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona (UAB), Barcelona, Spain.
MethodsX. 2023 Feb 6;10:102057. doi: 10.1016/j.mex.2023.102057. eCollection 2023.
Plastic pollution is a global problem. Animals and humans can ingest and inhale plastic particles, with uncertain health consequences. Nanoplastics (NPs) are particles ranging from 1 nm to 1000 nm that result from the erosion or breakage of larger plastic debris, and can be highly polydisperse in physical properties and heterogeneous in composition. Potential effects of NPs exposure may be associated with alterations in the xenobiotic metabolism, nutrients absorption, energy metabolism, cytotoxicity, and behavior. In humans, no data on NPs absorptions has been reported previously. Given that their detection relies significantly on environmental exposure, we have prospectively studied the presence of NPs in human peripheral blood (PB). Specifically, we have used fluorescence techniques and nanocytometry, together with the staining of the lipophilic dye Nile Red (NR), to demonstrate that NPs can be accurately detected using flow cytometry.•Potential effects of nanoplastics exposure.•Fluorescence techniques and nanocytometry.•Accurate detection using flow cytometry.
塑料污染是一个全球性问题。动物和人类会摄入和吸入塑料颗粒，其对健康的影响尚不确定。纳米塑料（NPs）是由较大塑料碎片的侵蚀或破碎产生的粒径范围为1纳米至1000纳米的颗粒，其物理性质可能高度多分散，成分也不均匀。接触纳米塑料的潜在影响可能与异生物质代谢、营养吸收、能量代谢、细胞毒性和行为的改变有关。在人类中，此前尚未有关于纳米塑料吸收情况的数据报道。鉴于纳米塑料的检测很大程度上依赖于环境暴露，我们前瞻性地研究了人类外周血（PB）中纳米塑料的存在情况。具体而言，我们使用了荧光技术和纳米细胞计数法，以及亲脂性染料尼罗红（NR）染色，以证明使用流式细胞术可以准确检测纳米塑料。
•纳米塑料暴露的潜在影响。
•荧光技术和纳米细胞计数法。
•使用流式细胞术进行准确检测。
